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Figure S1. Relative Mkc1 and Cek1 activation levels in glucose- and lactate-grown RM1000 cells as revealed by western blotting with phospho-specific antibodies. (A) and (B) show two independent experiments which are representative of six biological replicates.

Figure S2. TEM images of C. albicans RM1000 cells grown on glucose plus lactate showing trails of mannan fibrils between cells (scale bar, 0.2 μm). This phenotype was observed in at least half of the cells in the section, n>50.

Figure S3. Significant phenotypes observed in response to cell wall stress. The resistance of selected mutants to Calcofluor White (A, 200 μg/ml) and Congo Red (B, 300 μg/ml) were analysed. Control plates without stress are shown on the left panels. The appropriate parental control strain is displayed above each set of mutants (blue outline). Carbon source dependent phenotypes that fit or do not fit the predictions based on differential protein expression patterns are marked with ticks or “x”, respectively. Only consistent differences between wild type and mutant strains are shown, pictures being representative of three independent experiments.

Table S1. Relative quantification of the wall proteins of C. albicans cells grown in glucose, lactate or a mix of the two

Table S2. Relative quantification of the secretome of C. albicans cells grown in glucose, lactate or a mix of the two

Table S3. Main functional categories of proteins regulated by carbon source

Table S4. List of all C. albicans strains used in this study.

Table S5. Detailed information on peptide identifications of the wall proteome.

Table S6. Detailed information on peptide identifications of the secretome.

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