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Identification of cell wall proteins in the flax (Linum usitatissimum) stem

Authors

  • Arnaud Day,

    1. Université de Lille 1 Sciences et Technologies and Protéomique, IFR 147, Villeneuve d'Ascq, France
    2. Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), INRA UMR 1281, Villeneuve d'Ascq, France
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  • Stéphane Fénart,

    1. Université de Lille 1 Sciences et Technologies and Protéomique, IFR 147, Villeneuve d'Ascq, France
    2. Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), INRA UMR 1281, Villeneuve d'Ascq, France
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  • Godfrey Neutelings,

    1. Université de Lille 1 Sciences et Technologies and Protéomique, IFR 147, Villeneuve d'Ascq, France
    2. Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), INRA UMR 1281, Villeneuve d'Ascq, France
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  • Simon Hawkins,

    1. Université de Lille 1 Sciences et Technologies and Protéomique, IFR 147, Villeneuve d'Ascq, France
    2. Stress Abiotiques et Différenciation des Végétaux Cultivés (SADV), INRA UMR 1281, Villeneuve d'Ascq, France
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  • Christian Rolando,

    1. Université de Lille 1 Sciences et Technologies and Protéomique, IFR 147, Villeneuve d'Ascq, France
    2. Miniaturisation pour la Synthèse, l'Analyse & la Protéomique (MSAP), USR CNRS 3290, Villeneuve d'Ascq, France
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  • Caroline Tokarski

    Corresponding author
    1. Miniaturisation pour la Synthèse, l'Analyse & la Protéomique (MSAP), USR CNRS 3290, Villeneuve d'Ascq, France
    • Université de Lille 1 Sciences et Technologies and Protéomique, IFR 147, Villeneuve d'Ascq, France
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  • Colour Online: See the article online to view Figs. 1 and 2 in colour.

Correspondence: Dr. Caroline Tokarski, Miniaturisation pour la Synthèse, l'Analyse & la Protéomique (MSAP), USR CNRS 3290, Université de Lille 1 Sciences et Technologies, 59655 Villeneuve d'Ascq Cedex, France

E-mail: Caroline.Tokarski@univ-lille1.fr.

Fax: +33 3 20 43 43 63

Abstract

Sequential salt (CaCl2, LiCl) extractions were used to obtain fractions enriched in cell wall proteins (CWPs) from the stem of 60-day-old flax (Linum usitatissimum) plants. High-resolution FT-ICR MS analysis and the use of recently published genomic data allowed the identification of 11 912 peptides corresponding to a total of 1418 different proteins. Subcellular localization using TargetP, Predotar, and WoLF PSORT led to the identification of 152 putative flax CWPs that were classified into nine different functional classes previously established for Arabidopsis thaliana. Examination of different functional classes revealed the presence of a number of proteins known to be involved in, or potentially involved in cell-wall metabolism in plants. The flax stem cell wall proteome was also compared with transcriptomic data previously obtained on comparable samples. This study represents a major contribution to the identification of CWPs in flax and will lead to a better understanding of cell wall biology in this species.

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