Citrulline enhances myofibrillar constituents expression of skeletal muscle and induces a switch in muscle energy metabolism in malnourished aged rats

Authors

  • Cécile Faure,

    1. Département de Biologie Expérimentale, Métabolique et Clinique (EA 4466), Faculté de pharmacie, Université Paris Descartes, Paris, France
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  • Béatrice Morio,

    1. INRA, UMR 1019, UNH, CRNH Auvergne, Université d'Auvergne, Unité de Nutrition Humaine, Clermont-Ferrand, France
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  • Philippe Chafey,

    1. Plateforme de Protéomique 3P5, Université Paris Descartes, Sorbonne Paris Cité, France
    2. Institut Cochin, Université Paris Descartes, INSERM U1016, CNRS, Paris, France
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  • Servane Le Plénier,

    1. Département de Biologie Expérimentale, Métabolique et Clinique (EA 4466), Faculté de pharmacie, Université Paris Descartes, Paris, France
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  • Philippe Noirez,

    1. Département de Biologie Expérimentale, Métabolique et Clinique (EA 4466), Faculté de pharmacie, Université Paris Descartes, Paris, France
    2. UFR STAPS, Université Paris Descartes, Sorbonne Paris Cité, Paris, France
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  • Voahangy Randrianarison-Huetz,

    1. Institut Cochin, Université Paris Descartes, INSERM U1016, CNRS, Paris, France
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  • Luc Cynober,

    1. Département de Biologie Expérimentale, Métabolique et Clinique (EA 4466), Faculté de pharmacie, Université Paris Descartes, Paris, France
    2. Service interhospitalier de Biochimie, Hôpitaux Cochin et Hôtel Dieu, AP-HP, Paris, France
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  • Christian Aussel,

    1. Département de Biologie Expérimentale, Métabolique et Clinique (EA 4466), Faculté de pharmacie, Université Paris Descartes, Paris, France
    2. Service de Biologie, Hôpital Emile Roux, AP-HP, Paris, France
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  • Christophe Moinard

    Corresponding author
    • Département de Biologie Expérimentale, Métabolique et Clinique (EA 4466), Faculté de pharmacie, Université Paris Descartes, Paris, France
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  • Colour Online: See the article online to view Fig. 3 in colour.

Correspondence: Dr. Christophe Moinard, Département de Biologie Expérimentale, Métabolique et Clinique, Faculté de Pharmacie, Université Paris Descartes, 4 avenue de l'Observatoire, 75270 Paris Cedex 06, France

E-mail: christophe.moinard@parisdescartes.fr

Fax: +33-1-53-73-97-56

Abstract

Citrulline (Cit) actions on muscle metabolism remain unclear. Those latter were investigated using a proteomic approach on Tibialis muscles from male Sprague-Dawley rats. At 23 months of age, rats were either fed ad libitum (AL group) or subjected to dietary restriction for 12 weeks. At the end of the restriction period, one group of rats was euthanized (R group) and two groups were refed for one week with a standard diet supplemented with nonessential amino acids group or Cit (CIT group). Results of the proteomic approach were validated using targeted Western blot analysis and assessment of gene expression of the related genes. Maximal activities of the key enzymes involved in mitochondrial functioning were also determined. Cit supplementation results in a significant increase in the protein expression of the main myofibrillar constituents and of a few enzymes involved in glycogenolysis and glycolysis (CIT vs. AL and R, p < 0.05). Conversely, the expression of oxidative enzymes from Krebs cycle and mitochondrial respiratory chain was significantly decreased (CIT vs. AL, p < 0.05). However, maximal activities of key enzymes of mitochondrial metabolism were not significantly affected, except for complex 1 which presented an increased activity (CIT vs. AL and R, p < 0.05). In conclusion, Cit supplementation increases expression of the main myofibrillar proteins and seems to induce a switch in muscle energy metabolism, from aerobia toward anaerobia.

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