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Figure S1. Selected senescence markers. (A) SAHF formations in OIS cells. (B) Upregulation of P16INK4A revealed by western blotting analysis.

Figure S2. Histone H4 peptide (amino acid 3-19) showed decreased acetylation level in senescent cells.

Figure S3. The protein expression level of HDAC1 and HDAC2 did not change in OIS cells.

Figure S4. Histone H3 K79 and K9 methylation levels did not change significantly in OIS cells.

Figure S5. Experimental design of SILAC.

Figure S6. STRING display of randomly picked proteins (generated by random number generator in Excel) showed no aggregation of protein-protein interaction modules.

Figure S7. Three selected SILAC spectra of upregulated mitochondrial proteins with accompanying ratios of the “light” senescent peptides to the “heavy” control peptides. The table contains the upregulated mitochondrial / oxidative phosphorylation proteins that were found in at least two of the replicates.

pmic7509-sup-0002-tableS1.xlsx15KTable S1.
pmic7509-sup-0003-tableS2.xlsx290KTable S2.
pmic7509-sup-0004-tableS3.xlsx29KTable S3.
pmic7509-sup-0005-tableS4.xlsx13KTable S4.

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