Common versus noble Bacillus subtilis differentially responds to air and argon gas plasma
Article first published online: 7 AUG 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 13, Issue 17, pages 2608–2621, September 2013
How to Cite
Winter, T., Bernhardt, J., Winter, J., Mäder, U., Schlüter, R., Weltmann, K.-D., Hecker, M. and Kusch, H. (2013), Common versus noble Bacillus subtilis differentially responds to air and argon gas plasma. Proteomics, 13: 2608–2621. doi: 10.1002/pmic.201200343
- Issue published online: 2 SEP 2013
- Article first published online: 7 AUG 2013
- Accepted manuscript online: 21 JUN 2013 01:45PM EST
- Manuscript Accepted: 3 JUN 2013
- Manuscript Revised: 19 APR 2013
- Manuscript Received: 3 AUG 2012
- Federal Ministry of Education and Research. Grant Numbers: PtJ-BIO/ 0315784A;, 03Z1CN21, 13N9320, 03Z2DN12
- German Research Foundation. Grant Number: SFB/TRR34
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Figure 1. Influence on the number of CFU´s for both plasma experiments. Cells were grown aerobically in LB medium at 37°C. CFU's samples were taken at four different timepoints. The first sample was taken at an OD540 0.5, right before the argon (5 W) and the air plasma (7.4 W) treatment. Right after as well as 60 min and 120 min after the plasma treatments, the remaining samples were taken and plated on LB plates. After incuabation overnight at 37°C, the colonies were counted. Each experiment was performed twice and differences between the biological replicates are indicated by error bars.
Table 1. Plasma expansion on the cylinder and total averaged plasma treatment time for one single bacterium assuming a perfect mixing of the suspension for three different supply voltages.
Table 2. Proteins with changed protein amount (proteome data) after argon plasma and air plasma treatment as well as for the control conditions (argon gas vs. air). Functional classification was derived on the base of SubtiWiki. The significance of spot volume differences of two-fold or higher was assessed by the Student's t test (α<0.01; Delta 2D “statistics” table, TMEV plugin) .
Table 3. Identification of proteins with changed protein amount (proteome data) after air plasma treatment as well as for the control conditions (argon gas vs. air). For identifications of proteins with changed protein amount after argon plasma treatment- please see [Winter et al.]
Table 4. List of altered genes after argon plasma treatment (2.5 W) and air plasma treatment (7.4 W). Functional classifications were derived on the base of SubtiWiki (ratio <0.5; >2; p-value 0.01).
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