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Integrative analysis of proteomic and transcriptomic data for identification of pathways related to simvastatin-induced hepatotoxicity
Article first published online: 12 APR 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Special Issue: FOCUS ON INTEGRATIVE PROTEOMICS
Volume 13, Issue 8, pages 1257–1275, April 2013
How to Cite
Cho, Y.-E., Moon, P.-G., Lee, J.-E., Singh, T. S. K., Kang, W., Lee, H.-C., Lee, M.-H., Kim, S.-H. and Baek, M.-C. (2013), Integrative analysis of proteomic and transcriptomic data for identification of pathways related to simvastatin-induced hepatotoxicity. Proteomics, 13: 1257–1275. doi: 10.1002/pmic.201200368
- Issue published online: 12 APR 2013
- Article first published online: 12 APR 2013
- Accepted manuscript online: 16 JAN 2013 05:40AM EST
- Manuscript Accepted: 6 NOV 2012
- Manuscript Revised: 10 OCT 2012
- Manuscript Received: 14 AUG 2012
- Kyungpook National University Research
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Figure S1. E.coli Protein and peptide numbers for the 1D and 2D-3 fraction E.coli experiments.
Figure S2. The Venn diagram of the overlap of E.coli proteins and peptides numbers from 1D-LC (A) and 2D-LC (B) analysis between two biological replicates, respectively.
Figure S3. Comparison of subcellular localization from proteins identified by gel-assisted digestion and in-solution digestion, respectively.
Figure S4. Treatment with simvastatin resulted in dose-dependent diminishment of viability of rat primary cultured hepatocytes (A).
Figure S5. The numbers of proteins and peptides identified from 1D-LC and 2D-LC methods (A), the Venn diagram of the overlap of proteins identified from 1-LC and 2D-LC (B).
Figure S6. 2D-5fraction control (A) and simvastatin-treated (B) rat primary hepatocyte separation.
Figure S7. Subcellular localization (A) and functional categorization (B) of differentially expressed genes in simvastatin-induced hepatotoxicity.
Figure S8. Correlation between protein and corresponding transcript modulation. Results presented all common proteins and genes (A) and differentially expressed proteins and genes (B).
Figure S9. A boxplot of the ranking of differentially expressed genes (DEGs) and differentially expressed proteins (DEPs).
Figure S10. mRNA expressions of HMG-COA reductase (HMG-COAR), and low density lipoprotein receptor (LDLR) in simvastatin-treated hepatocytes.
|pmic7353-sup-0002-TableS1.xls||7133K||Table S1. List of proteins identified from twelve runs of control and simvastatin-treated groups.|
|pmic7353-sup-0003-TableS2.pdf||20K||Table S2. Gene specific primers used in real-time PCR confirmation experiments.|
|pmic7353-sup-0004-TableS3.xls||69K||Table S3. mRNA changes in simvastatin-treated hepatocytes at p < 0.05 and fold change greater than 2.0 or less than 0.5.|
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