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Quantitative proteomics of tomato defense against Pseudomonas syringae infection
Article first published online: 27 APR 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 13, Issue 12-13, pages 1934–1946, June 2013
How to Cite
Parker, J., Koh, J., Yoo, M.-J., Zhu, N., Feole, M., Yi, S. and Chen, S. (2013), Quantitative proteomics of tomato defense against Pseudomonas syringae infection. Proteomics, 13: 1934–1946. doi: 10.1002/pmic.201200402
- Issue published online: 5 JUL 2013
- Article first published online: 27 APR 2013
- Accepted manuscript online: 27 MAR 2013 02:59AM EST
- Manuscript Accepted: 8 FEB 2013
- Manuscript Revised: 17 JAN 2013
- Manuscript Received: 31 AUG 2012
- University of Florida and the National Institute of Health. Grant Number: 1S10RR025418–01
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|pmic7418-sup-0001-FgureS1.pptx||80K||Figure S1. Design of iTRAQ labeling and analysis. All eight iTRAQ tags were used to label the Pst and mock inoculated samples. After LC-MS/MS, resulting protein expression was analyzed against its control. This information was then used to examine differences between time points and genotypes.|
|pmic7418-sup-0002-FgureS2.pptx||827K||Figure S2. DAB staining time course. (A) PtoR mock treated and inoculated with Pst. (a) PtoR, mock 0hai; (b) PtoR, mock 2hai; (c) PtoR, mock 4hai; (d) PtoR, mock 6hai; (e) PtoR, mock 8hai; (f) PtoR, mock 24hai; (g) PtoR, mock 48hai; (h) PtoR, Pst 0hai; (i) PtoR, Pst 2hai; (j) PtoR, Pst 4hai; (k) PtoR, Pst 6hai; (l) PtoR, Pst 8hai; (m) PtoR, Pst 24hai; (n) PtoR, Pst 48hai. (B) prf3 mock trated and inoculated with Pst. (a) prf3, mock 0hai; (b) prf3, mock 2hai; (c) prf3, mock 4hai; (d) prf3, mock 6hai; (e) prf3, mock 8hai; (f) prf3, mock, 24hai; (g) prf3, mock 48hai; (h) prf3, Pst 0hai; (i) prf3, Pst 2hai; (j) prf3, Pst 4ha; (k) prf3, Pst 6hai; (l) prf3, Pst 8hai; (m) prf3, Pst 24hai; (n) prf3, Pst 48hai.|
|pmic7418-sup-0003-FgureS3.pptx||863K||Figure S3. Representative peptide sequencing and quantification result showing the levels of pathogenesis-related protein (PR-10) in different samples. (A) Table of b and y ions identified the peptide sequence from PR-10 protein; (B) MS/MS spectrum with b and y ions annotated. The red arrow indicates the cluster of iTRAQ tags; (C) Zoomed in view of the iTRAQ tags indicated in B). The peak intensity correlates to the level of PR-10 protein in each sample shown above the corresponding tag.|
|pmic7418-sup-0004-TableS1.xlsx||2919K||Table S1. Confident proteins with an unused score of 1.3 or greater. Significant proteins with a change in expression are highlighted in green for increase (> 1.2), red for decrease (< 0.8), and yellow for having been identified in different comparisons with an increase and decrease. Accession numbers and protein names are annotated as provided by the Harvaard database. Protein expression values are listed under the relative tag ratios. Tag ratios are composed of the treated sample over the relative control. P-values are listed for statistical significance evaluation.|
|pmic7418-sup-0005-TableS2.xlsx||11K||Table S2. List of Q-RT PCR Primers. Proteins chosen for Q-RT PCR are listed along with the sequence which is observed. Proteins were annotated using Blast2Go annotations.|
|pmic7418-sup-0006-TableS3.xlsx||222K||Table S3. Comparisons between genotypes and time points. Proteins with an increase, decrease, and differential expression were examined between genotype and time point combinations. Comparisons include PtoR4hai and PtoR 24hai, prf3 4hai and prf3 24hai, PtoR4hai and prf3 4hai, and PtoR24hai and prf3 24hai. Proteins in this table are significant with an increase or decrease in expression. Expression values and p-values (p-value < 0.05) are listed. The biological process the protein was annotated as being involved is listed beside the protein. Proteins were annotated using Blast2Go software.|
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