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Evaluation of three-dimensional gel electrophoresis to improve quantitative profiling of complex proteomes

Authors


Correspondence: Dr. Sergio Mauro, Département Sciences du Vivant, Centre wallon de Recherches agronomiques, chaussée de Charleroi 234, B-5030 Gembloux, Belgium

E-mail: mauro@cra.wallonie.be

Fax: +32-081-627-399

Abstract

Two-dimensional remains one of the main experimental approaches in proteome analysis. However, comigration of protein leads to several limitations: lack of accuracy in protein identification, impaired comparative quantification, and PTM detection. We have optimized a third additional step of in-gel separation to alleviate comigration associated drawbacks. Spot resolution is strikingly improved following this simple and rapid method and the positive impact on protein and peptide identification from MS/MS data, on the analysis of relative changes in protein abundance, and on the detection of PTM is described.

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