These authors contributed equally to this work.
Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells
Version of Record online: 22 JUL 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 13, Issue 16, pages 2377–2385, August 2013
How to Cite
Zeng, L., Sun, Y., Xie, L., Wei, L., Ren, Y., Zhao, J., Qin, W., Mitchelson, K. and Cheng, J. (2013), Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells. Proteomics, 13: 2377–2385. doi: 10.1002/pmic.201200521
- Issue online: 9 AUG 2013
- Version of Record online: 22 JUL 2013
- Accepted manuscript online: 10 JUN 2013 01:40AM EST
- Manuscript Accepted: 11 MAY 2013
- Manuscript Revised: 14 MAR 2013
- Manuscript Received: 18 NOV 2012
- National Natural Science Foundation of China. Grant Number: 31100983
- National Hi-Tech Program of China. Grant Number: 2006AA020701
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Figure S1. Microwell colorimetric TF assay shows the amounts of active (DNA binding) USF2 and STAT1 increased in mouse fibroblast cell lines following IFN-γ stimulation.
Table S1. Targeted immuno-co-precipitation oligonucleotide array-based transcription factor array (TIC-OATFA) assay results for STAT1. STAT1 was co-precipitated along with seven other TFs: USF1, USF2, nuclear factor of activated T cells (NFAT), TBP, NFE2, NFκB, and NF1.
Table S2. Biotin-labeled probes for microwell colorimetric transcription factor assays. This assay verifies the binding activity of STAT1 and USF2 in fibroblast NE with STAT1/USF2-specific DNA sequences.
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