Functional proteomics reveals the protective effects of saffron ethanolic extract on hepatic ischemia-reperfusion injury

Authors

  • Tai-Long Pan,

    Corresponding author
    1. Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan
    • School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Tung-Ho Wu,

    1. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan
    2. Division of Cardiovascular Surgery, Veterans General Hospital, Kaohsiung, Taiwan
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Pei-Wen Wang,

    1. School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan
    Search for more papers by this author
  • Yann-Lii Leu,

    1. Chinese Herbal Medicine Research Team, Healthy Aging Research Center, Chang Gung University, Taoyuan, Taiwan
    2. Graduate Institute of Natural Products, Chang Gung University, Taoyuan, Taiwan
    Search for more papers by this author
  • Nardnisa Sintupisut,

    1. Institute of Biological Chemistry, Academy Sinica, Taipei, Taiwan
    Search for more papers by this author
  • Chun-Hsun Huang,

    1. Research Center for Industry of Human Ecology, Chang Gung University of Science and Technology, Taoyuan, Taiwan
    Search for more papers by this author
  • Fang-Rong Chang,

    1. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan
    Search for more papers by this author
  • Yang-Chang Wu

    Corresponding author
    1. Graduate Institute of Natural Products, Kaohsiung Medical University, Kaohsiung, Taiwan
    2. Graduate Institute of Integrated Medicine, College of Chinese Medicine, China Medical University, Taichung, Taiwan
    3. Natural Medicinal Products Research Center and Center for Molecular Medicine, China Medical University Hospital, Taichung, Taiwan
    • School of Traditional Chinese Medicine, Chang Gung University, Taoyuan, Taiwan
    Search for more papers by this author

  • Colour Online: See the article online to view Figs. 1–7 in colour.

Correspondence: Dr. Tai-Long Pan, School of Traditional Chinese Medicine, Chang Gung University, 259 Wen-Hwa 1st Road, Kweishan, Taoyuan 333, Taiwan

E-mail: pan@mail.cgu.edu.tw

Fax: +886-3-211-8700

Additional corresponding author: Professor Yang-Chang Wu,

E-mail: yachwu@mail.cmu.edu.tw

Abstract

Hepatic ischemia-reperfusion (IR) injury is a common clinical problem and ROS may be a contributing factor on IR injury. The current study evaluates the potential protective effect of saffron ethanol extract (SEE) in a rat model upon hepatic IR injury. Caspases 3 and terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end labeling (TUNEL) results showed increased cell death in the IR samples; reversely, minor apoptosis was detected in the SEE/IR group. Pretreatment with SEE significantly restored the content of antioxidant enzymes (SOD1 and catalase) and remarkably inhibited the intracellular ROS concentration in terms of reducing p47phox translocation. Proteome tools revealed that 20 proteins were significantly modulated in protein intensity between IR and SEE/IR groups. Particularly, SEE administration could attenuate the carbonylation level of several chaperone proteins. Network analysis suggested that saffron extract could alleviate IR-induced ER stress and protein ubiquitination, which finally lead to cell apoptosis. Taken together, SEE could reduce hepatic IR injury through modulating protein oxidation and our results might help to develop novel therapeutic strategies against ROS-caused diseases.

Ancillary