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Figure S1. LC-UV chromatogram of saffron. Mobile phase of buffer A: 1% acetic acid-water and buffer B: 1% acetic acid-acetonitrile. The initial condition was set at 10% of B, grading up to 20% in 15 min, up to 20% at 20 min and up to 40% at 50 min. Detection wavelength was 310 nm. Oven temperature was 30°C and flow rate was 0.5 mL/min. The chemical structures of marked peaks are illustrated.

Figure S2. Validation of p47 translocation via immunohistochemical study. The positive signal was presented as brown color and indicated by arrows. Original magnification: 400x

Figure S3. MALDI-TOF spectra of spot 5 was matched the rat SOD1 protein from the Swiss-Prot database (2012_05). The parent ions m/z 1367.7710 was selected for further analysis by an Ultraflex™ MS/MS operated in the LIFT mode using FlexControl™ software. A sequence was confirmed from the labeled b- and y-ions in the spectrum.

Figure S4. Biological network analyses of differentially expressed proteins using MetaCore™ mapping tools. Nodes represent proteins and lines between the nodes indicate direct protein–protein interactions. The related biologic processes in this network are mainly involved in ER stress and the ubiquitin-proteasome system. The various proteins on this map are indicated by different symbols representing the functional class of the proteins.

pmic7469-sup-0002-suppmat.doc25Ksupplementary material

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