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In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine

Authors

  • Simona Principe,

    1. Ontario Cancer Institute, University Health Network, Toronto, ON, Canada
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    • These three authors contributed equally to the work.

  • E. Ellen Jones,

    1. Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA
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    • These three authors contributed equally to the work.

  • Yunee Kim,

    1. Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada
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    • These three authors contributed equally to the work.

  • Ankit Sinha,

    1. Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada
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  • Julius O. Nyalwidhe,

    1. Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA
    2. The Leroy T. Canoles Jr., Cancer Research Center, Eastern Virginia Medical School, Norfolk, VA, USA
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  • Jasmin Brooks,

    1. Ontario Cancer Institute, University Health Network, Toronto, ON, Canada
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  • O. John Semmes,

    1. Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada
    2. Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA
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  • Dean A. Troyer,

    1. Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA
    2. The Leroy T. Canoles Jr., Cancer Research Center, Eastern Virginia Medical School, Norfolk, VA, USA
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  • Raymond S. Lance,

    1. The Leroy T. Canoles Jr., Cancer Research Center, Eastern Virginia Medical School, Norfolk, VA, USA
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  • Thomas Kislinger,

    1. Ontario Cancer Institute, University Health Network, Toronto, ON, Canada
    2. Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada
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  • Richard R. Drake

    Corresponding author
    1. Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA
    • Ontario Cancer Institute, University Health Network, Toronto, ON, Canada
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  • Colour Online: See the article online to view Figs. 1 and 2 in colour.

Correspondence: Dr. Richard R. Drake, Department of Cell and Molecular Pharmacology and Experimental Therapeutics, Medical University of South Carolina, Charleston, SC, USA

E-mail: draker@musc.edu

Abstract

Expressed prostatic secretions (EPS) are proximal fluids of the prostate that are increasingly being utilized as a clinical source for diagnostic and prognostic assays for prostate cancer (PCa). These fluids contain an abundant amount of microvesicles reflecting the secretory function of the prostate gland, and their protein composition remains poorly defined in relation to PCa. Using expressed prostatic secretions in urine (EPS-urine), exosome preparations were characterized by a shotgun proteomics procedure. In pooled EPS-urine exosome samples, ∼900 proteins were detected. Many of these have not been previously observed in the soluble proteome of EPS generated by our labs or other related exosome proteomes. We performed systematic comparisons of our data against previously published, prostate-related proteomes, and global annotation analyses to highlight functional processes within the proteome of EPS-urine derived exosomes. The acquired proteomic data have been deposited to the Tranche repository and will lay the foundation for more extensive investigations of PCa derived exosomes in the context of biomarker discovery and cancer biology.

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