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FilenameFormatSizeDescription
pmic7544-sup-0001-figureS1.tif7099KFigure 1. Sections of cerebellum (x 10) containing the molecular and granule layers stained with hematoxylin and eosin, following (a) sacrifice by microwave irradiation at 5kW for 0.94 s and (b) sacrifice by decapitation. Several ‘scratches’, those were made during cryosectioning were found in FMW-treated sections due to its fragile property. However, the nucleus structures were intact and interfaces among molecular layer, granule layer and white matter were clearly defined. W: white matter, ML: molecular layer, GL: granule layer.
pmic7544-sup-0002-figureS2.tif1028KFigure 2. Mass spectra obtained from the brain sections those were treated with FMW, ISF and PEF (1min). The mass peaks corresponding to metabolites whose distributions were visualized in Fig. 2 were highlighted.
pmic7544-sup-0003-figureS3.tif853KFigure 3. Mass spectra obtained from the brain sections prepared from non-labeled (green) and 13C6-glucose administered mice (red). The mass peaks corresponding to non-labeled and 13C-labled compounds of glutamate and UDP-glucose were highlighted. Their brain distributions were visualized in Fig. 4.
pmic7544-sup-0004-supmat.doc1074KTable 1. Absolute concentrations of brain metabolites measured by CE/ESI-MS prepared by the four different sampling methods.

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