Characterization of a high field Orbitrap mass spectrometer for proteome analysis
Version of Record online: 30 JUL 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 13, Issue 17, pages 2552–2562, September 2013
How to Cite
Pachl, F., Ruprecht, B., Lemeer, S. and Kuster, B. (2013), Characterization of a high field Orbitrap mass spectrometer for proteome analysis. Proteomics, 13: 2552–2562. doi: 10.1002/pmic.201300076
- Issue online: 2 SEP 2013
- Version of Record online: 30 JUL 2013
- Accepted manuscript online: 8 JUL 2013 04:56AM EST
- Manuscript Accepted: 26 JUN 2013
- Manuscript Revised: 8 MAY 2013
- Manuscript Received: 22 FEB 2013
- Collaborative Research Center
- Technische Universität München, Germany
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Figure S1: HCD parameter optimization. All parameters shown were optimized using 1 ug E.coli digest and a 60’ LC gradient time. The evaluation criteria included the number of identified spectra, peptides and proteins.
A) Collision energy (in %)
B) Automatic gain control settings (number of collected precursor ion charges for fragmentation)
C) number of most abundant precursor ions picked for fragmentation in one cycle
D) Signal threshold of precursor ions for triggering an MS/MS spectrum
E) Survey scan start m/z values
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