Comparative proteomic analysis of methyl jasmonate-induced defense responses in different rice cultivars

Authors

  • Yunfeng Li,

    1. Laboratory of Physiological Plant Pathology, South China Agricultural University, Guangzhou, P. R. China
    2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, P. R. China
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Yanfang Nie,

    1. College of Natural Resources and Environment, South China Agricultural University, Guangzhou, P. R. China
    Search for more papers by this author
    • These authors contributed equally to this work.

  • Zhihui Zhang,

    1. Laboratory of Physiological Plant Pathology, South China Agricultural University, Guangzhou, P. R. China
    2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, P. R. China
    Search for more papers by this author
  • Zhijian Ye,

    1. Laboratory of Physiological Plant Pathology, South China Agricultural University, Guangzhou, P. R. China
    2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, P. R. China
    Search for more papers by this author
  • Xiaotao Zou,

    1. Laboratory of Physiological Plant Pathology, South China Agricultural University, Guangzhou, P. R. China
    2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, P. R. China
    Search for more papers by this author
  • Lianhui Zhang,

    1. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, P. R. China
    Search for more papers by this author
  • Zhenzhong Wang

    Corresponding author
    1. Laboratory of Physiological Plant Pathology, South China Agricultural University, Guangzhou, P. R. China
    2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, P. R. China
    • Correspondence: Dr. Zhenzhong Wang, Laboratory of Physiological Plant Pathology, South China Agricultural University, Guangzhou City, 510642, P. R. China

      E-mail: zzwang@scau.edu.cn

      Fax: 86-20-85281107

    Search for more papers by this author

  • Colour Online: See the article online to view Figs. 1 and 2 in colour.

Abstract

Jasmonate is an important endogenous chemical signal that plays a role in modulation of plant defense responses. To understand its mechanisms in regulation of rice resistance against the fungal pathogen Magnaporthe oryzae, comparative phenotype and proteomic analyses were undertaken using two near-isogenic cultivars with different levels of disease resistance. Methyl-jasmonate (MeJA) treatment significantly enhanced the resistance against M. oryzae in both cultivars but the treated resistant cultivar maintained a higher level of resistance than the same treated susceptible cultivars. Proteomic analysis revealed 26 and 16 MeJA-modulated proteins in resistant and susceptible cultivars, respectively, and both cultivars shared a common set of 13 proteins. Cumulatively, a total of 29 unique MeJA-influenced proteins were identified with many of them known to be associated with plant defense response and ROS accumulation. Consistent with the findings of proteomic analysis, MeJA treatment increased ROS accumulation in both cultivars with the resistant cultivar showing higher levels of ROS production and cell membrane damage than the susceptible cultivar. Taken together, our data add a new insight into the mechanisms of overall MeJA-induced rice defense response and provide a molecular basis of using MeJA to enhance fungal disease resistance in resistant and susceptible rice cultivars.

Ancillary