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Amino-functionalized macroporous silica for efficient tryptic digestion in acidic solutions

Authors

  • Jinrui Gan,

    1. Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China
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  • Kun Qian,

    1. Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China
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  • Jingjing Wan,

    1. Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China
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  • Liang Qiao,

    1. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
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  • Weichao Guo,

    1. Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China
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  • Pengyuan Yang,

    1. Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China
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  • Hubert H. Girault,

    1. Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland
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  • Baohong Liu

    Corresponding author
    1. Department of Chemistry, Institute of Biomedical Sciences and State Key Lab of Molecular Engineering of Polymers, Fudan University, Shanghai, China
    • Correspondence: Professor Baohong Liu, Department of Chemistry, Fudan University, Shanghai 200433, China

      E-mail: bhliu@fudan.edu.cn

      Fax: +86-21-65641740

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Abstract

Amino-functionalized macroporous silica foam (NH2-MOSF) has been developed as a host reactor to realize highly efficient proteolysis in acidic solutions where normal tryptic reactions cannot occur. The digestion protocol consists simply of adding the functionalized NH2-MOSF into the protein and trypsin solutions without altering the bulk pH or preloading the enzymes on the materials. With this protocol, digestion of sample fractions from LC can be efficiently realized in the acidic solutions directly. Digestion of a protein fraction extracted from rat liver tissue after LC separation was performed to illustrate this principle, where 103 proteins were successfully identified at pH 3 after 1.5 h of tryptic digestion.

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