These authors contributed equally to this work.
Protein interactome analysis of 12 mitogen-activated protein kinase kinase kinase in rice using a yeast two-hybrid system
Article first published online: 13 JAN 2014
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 14, Issue 1, pages 105–115, January 2014
How to Cite
Singh, R., Lee, J.-E., Dangol, S., Choi, J., Yoo, R. H., Moon, J. S., Shim, J.-K., Rakwal, R., Agrawal, G. K. and Jwa, N.-S. (2014), Protein interactome analysis of 12 mitogen-activated protein kinase kinase kinase in rice using a yeast two-hybrid system. Proteomics, 14: 105–115. doi: 10.1002/pmic.201300125
Colour Online: See the article online to view Figs. 1–5 in colour.
- Issue published online: 13 JAN 2014
- Article first published online: 13 JAN 2014
- Accepted manuscript online: 18 NOV 2013 02:53AM EST
- Manuscript Accepted: 6 NOV 2013
- Manuscript Revised: 27 OCT 2013
- Manuscript Received: 27 MAR 2013
- Plant Molecular Breeding Center. Grant Number: PJ008061
- Rural Development Administration, Republic of Korea
- Ministry of Science, ICT and Future Planning. Grant Number: 2013R1A1A2009269
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|pmic7611-sup-0001-FigureS1.ppt||327K||Figure S1. Dendogram of 12 rice MAP3Ks proteins (baits used in this study) and other plants MAPKKK proteins. The phylogenetic tree was constructed by CLUSTAL W 2.0 program (http://www.ebi.ac.uk/clustalw2). Twelve MAP3Ks proteins were classified into 2 groups (MEKKs) and (Raf-like) as shown. Abbreviations: At, Arabidopsis thaliania; Bn, Brassica napus; Nb, Nicotiana benthamania; Le, Lycopersicon esculentum; Os, Oryzae sativa; Hv, Hordeum vulgare; Zm, Zea mays.|
|pmic7611-sup-0002-FigureS2.ppt||173K||Figure S2. Functional categorization of identified MAP3K IPPs. Identified MAP3K interacting proteins were classified into 20 functional categories based on the GO annotation, described in the TIGR database (http://rice.plantbiology.msu.edu/). Numbers on the top of each bar represent the total number of annotated interacting proteins.|
|pmic7611-sup-0003-FigureS3.ppt||548K||Figure S3. In vivo BiFC analysis of Y2H detected IPPs in onion epidermal cells. Onion epidermal cells were transformed with mixtures of interacting protein pairs (OsMEKK11 + OsGRF), (OsMEKK11 + OsEP2), (OsMEKK24 + OsFHA1) and (OsMEKK24 + OsFHA2) using a biolistic method as described in the Materials and methods section. Venus fluorescence and localization were observed by confocal laser microscopy. See text for details.|
|pmic7611-sup-0004-FigureS4.ppt||794K||Figure S4. In vivo BiFC analysis of negative controls co-expressing bait with empty pDEST-VYNE (R)GW and prey with empty pDEST-VYCE(R)GW in onion epidermal cells. A. MAP3Ks bait proteins were co-expressed with empty pDEST-VYNE(R)GW and analysed for venus signal by confocal microscopy. B. and C. MAP3K prey proteins were co-expressed with empty pDEST-VYCE(R)GW and analysed for venus signal by confocal microscopy. See text for details.|
|pmic7611-sup-0005-FigureS5.ppt||1373K||Figure S5. Localization analysis of individual interactor in onion epidermal cells. A. Localization of empty vector (pGWB552); B. Localization of OsMEKK8 and its interacting protein OsAPK1; C. Localization of OsMEKK11 and its interacting proteins OsEP2, OsGRF and OsPep; D. Localization of OsMEKK24 and its interactors OsFHA1 and OsFHA2; E. Localization of OsMEKK25 and its interactors OsEP1 and OsRingE3; F. Localization of OsMEKK55 and its interactors OsDegP10. p, plasma membrane; c, cytoplasm; and n, nucleus.|
|pmic7611-sup-0006-TableS1.xls||31K||Table S1. List of primers used in this study. (Excel)|
|pmic7611-sup-0007-TableS2.xls||37K||Table S2. List of rice MAP3K proteins used as baits (12 baits) for Y2H library screening. (Excel)|
|pmic7611-sup-0008-TableS3.xls||44K||Table S3. List of rice proteins used as baits (12 baits) for Y2H library screening with corresponding screened preys (12). (Excel)|
|pmic7611-sup-0009-TableS4.xlsx||12K||Table S4. Type of MAP3K interacting proteins summarized from studies done by  and .|
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