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FilenameFormatSizeDescription
pmic7611-sup-0001-FigureS1.ppt327KFigure S1. Dendogram of 12 rice MAP3Ks proteins (baits used in this study) and other plants MAPKKK proteins. The phylogenetic tree was constructed by CLUSTAL W 2.0 program (http://www.ebi.ac.uk/clustalw2). Twelve MAP3Ks proteins were classified into 2 groups (MEKKs) and (Raf-like) as shown. Abbreviations: At, Arabidopsis thaliania; Bn, Brassica napus; Nb, Nicotiana benthamania; Le, Lycopersicon esculentum; Os, Oryzae sativa; Hv, Hordeum vulgare; Zm, Zea mays.
pmic7611-sup-0002-FigureS2.ppt173KFigure S2. Functional categorization of identified MAP3K IPPs. Identified MAP3K interacting proteins were classified into 20 functional categories based on the GO annotation, described in the TIGR database (http://rice.plantbiology.msu.edu/). Numbers on the top of each bar represent the total number of annotated interacting proteins.
pmic7611-sup-0003-FigureS3.ppt548KFigure S3. In vivo BiFC analysis of Y2H detected IPPs in onion epidermal cells. Onion epidermal cells were transformed with mixtures of interacting protein pairs (OsMEKK11 + OsGRF), (OsMEKK11 + OsEP2), (OsMEKK24 + OsFHA1) and (OsMEKK24 + OsFHA2) using a biolistic method as described in the Materials and methods section. Venus fluorescence and localization were observed by confocal laser microscopy. See text for details.
pmic7611-sup-0004-FigureS4.ppt794KFigure S4. In vivo BiFC analysis of negative controls co-expressing bait with empty pDEST-VYNE (R)GW and prey with empty pDEST-VYCE(R)GW in onion epidermal cells. A. MAP3Ks bait proteins were co-expressed with empty pDEST-VYNE(R)GW and analysed for venus signal by confocal microscopy. B. and C. MAP3K prey proteins were co-expressed with empty pDEST-VYCE(R)GW and analysed for venus signal by confocal microscopy. See text for details.
pmic7611-sup-0005-FigureS5.ppt1373KFigure S5. Localization analysis of individual interactor in onion epidermal cells. A. Localization of empty vector (pGWB552); B. Localization of OsMEKK8 and its interacting protein OsAPK1; C. Localization of OsMEKK11 and its interacting proteins OsEP2, OsGRF and OsPep; D. Localization of OsMEKK24 and its interactors OsFHA1 and OsFHA2; E. Localization of OsMEKK25 and its interactors OsEP1 and OsRingE3; F. Localization of OsMEKK55 and its interactors OsDegP10. p, plasma membrane; c, cytoplasm; and n, nucleus.
pmic7611-sup-0006-TableS1.xls31KTable S1. List of primers used in this study. (Excel)
pmic7611-sup-0007-TableS2.xls37KTable S2. List of rice MAP3K proteins used as baits (12 baits) for Y2H library screening. (Excel)
pmic7611-sup-0008-TableS3.xls44KTable S3. List of rice proteins used as baits (12 baits) for Y2H library screening with corresponding screened preys (12). (Excel)
pmic7611-sup-0009-TableS4.xlsx12KTable S4. Type of MAP3K interacting proteins summarized from studies done by [12] and [13].

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