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pmic7682-sup-0001-FigureS1.tif2865KFigure S1 Normal distribution analysis of relative spot intensity changes between hybrid and its parental lines.
pmic7682-sup-0002-FigureS2.tif549KFigure S2 Quality analysis of RNA. 2 μg RNA per sample was used to agarose gels electrophoresis and yields were given in parentheses.
pmic7682-sup-0003-FigureS3.tif124KFigure S3 Stability evaluation of three housekeeping genes expression.
pmic7682-sup-0004-FigureS4.tif471KFigure S4 Efficiency tests for all gene primers using dilutions. Standard curve with the Ct plotted against the log of the starting quantity of template for each dilution. The efficiency, slope and the R value are shown right the graph.
pmic7682-sup-0005-FigureS5.tif160KFigure S5 DNA contamination checking in qRT-PCR analyses.
pmic7682-sup-0006-FigureS6.tif682KFigure S6 Melting curve analyses of the qRT-PCR products.
pmic7682-sup-0007-FigureS7.tif3907KFigure S7 Amplification curve analyses of the qRT-PCR products.
pmic7682-sup-0008-FigureS8.tif1671KFigure S8 Maize leaf nuclear proteome maps of hybrid Mo17/B73 and its parental lines Mo17, B73 at 5 DAG. The number of differentially expressed protein spots that were identified by MS was labeled on the map. The identified protein spots were summarized in Table 3.
pmic7682-sup-0009-FigureS9.tif217KFigure S9 Comparative analysis of maize leaf nuclear proteome and leaf global proteome. The data of leaf global proteome was derived from a previous study [11]. The diagrams showed the number of nuclear proteins (blue), leaf global proteins (yellow) and overlapped proteins (faint yellow). Detailed information is available in Supplementary Table S6.
pmic7682-sup-0010-FigureS10.tif899KFigure S10 Comparison of nuclear protein results in present study with maize leaf transcriptomic data from a previous study [10]. Detailed information is available in Supplementary Table S7.
pmic7682-sup-0011-FigureS11.tif358KFigure S11 Western blot analysis of H3ac level between maize hybrid and its parental lines. Histone H3 as an internal control.
pmic7682-sup-0012-TableS1.xls24KTable S1 Primers used in this study.
pmic7682-sup-0013-TableS2.xls361KTable S2 Amino acid sequences of peptides identified for each protein spot indicated.
pmic7682-sup-0014-TableS3.doc113KTable S3 Classification of the 68 identified protein isoforms derived from 29 proteins.
pmic7682-sup-0015-TableS4.xls22KTable S4 The result of power analysis for the differentially expressed proteins between maize hybrid and its parental lines.
pmic7682-sup-0016-TableS5.doc69KTable S5 Analysis of six genes expression by qRT-PCR.
pmic7682-sup-0017-TableS6.xls128KTable S6 Comparative analysis of identified proteins in maize leaf nuclear proteome and leaf total proteome.
pmic7682-sup-0018-TableS7.xls44KTable S7 K-means cluster categories of the maize leaf nuclear proteins.

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