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When 2D is not enough, go for an extra dimension

Authors

  • Thierry Rabilloud

    Corresponding author
    1. Univ. Grenoble Alpes, LCBM, Grenoble, France
    2. CEA, iRTSV/LCBM, Grenoble, France
    • CNRS, Laboratory of Chemistry and Biology of Metals (LCBM), Grenoble, France
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Correspondence: Dr. Thierry Rabilloud, UMR CNRS-CEA-UJF 5249, iRTSV/LCBM, CEA Grenoble, 17 rue des martyrs, 38054 Grenoble CEDEX 9, France

E-mail: thierry.rabilloud@cea.fr

Abstract

The use of an extra SDS separation in a different buffer system provide a technique for deconvoluting 2D gel spots made of several proteins (Colignon et al. Proteomics, 2013, 13, 2077–2082). This technique keeps the quantitative analysis of the protein amounts and combines it with a strongly improved identification process by mass spectrometry, removing identification ambiguities in most cases. In some favorable cases, posttranslational variants can be separated by this procedure. This versatile and easy to use technique is anticipated to be a very valuable addition to the toolbox used in 2D gel-based proteomics.

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