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Proteomic identification of rainbow trout seminal plasma proteins

Authors

  • Joanna Nynca,

    Corresponding author
    1. Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
    • Correspondence: Dr. Joanna Nynca, Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland

      E-mail: j.nynca@pan.olsztyn.pl

      Fax: +48-89-524-01-24

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  • Georg J. Arnold,

    1. Laboratory for Functional Genome Analysis (LAFUGA), Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany
    2. Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany
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  • Thomas Fröhlich,

    1. Laboratory for Functional Genome Analysis (LAFUGA), Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany
    2. Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany
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  • Kathrin Otte,

    1. Laboratory for Functional Genome Analysis (LAFUGA), Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany
    2. Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany
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  • Florian Flenkenthaler,

    1. Laboratory for Functional Genome Analysis (LAFUGA), Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany
    2. Gene Center and Department of Biochemistry, Ludwig-Maximilians-Universität, Munich, Germany
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  • Andrzej Ciereszko

    1. Department of Gametes and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland
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  • Colour Online: See the article online to view Figs. 1 and 2 in colour.

Abstract

In the study, the combination of protein fractionation by 1DE and HPLC-ESI-MS/MS was used to characterize the rainbow trout seminal plasma proteome. Our results led to the creation of a catalogue of rainbow trout seminal plasma proteins (152 proteins) and significantly contributed to the current knowledge regarding the protein composition of fish seminal plasma. The major proteins of rainbow trout seminal plasma, such as transferrin, apolipoproteins, complement C3, serum albumin, and hemopexin-, alpha-1-antiproteinase-, and precerebellin-like protein, were recognized as acute-phase proteins (proteins that plasma concentration changes in response to inflammation). This study provides the basis for further functional studies of fish seminal plasma proteins, as well as for the identification of novel biomarkers for sperm quality. The MS data have been deposited in the ProteomeXchange with identifier PXD000306 (http://proteomecentral.proteomexchange.org/dataset/PXD000306).

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