Comprehensive proteomic datasets for studying adipocyte–macrophage cell–cell communication
Article first published online: 4 DEC 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 13, Issue 23-24, pages 3424–3428, December 2013
How to Cite
Freiwald, A., Weidner, C., Witzke, A., Huang, S.-Y., Meierhofer, D. and Sauer, S. (2013), Comprehensive proteomic datasets for studying adipocyte–macrophage cell–cell communication. Proteomics, 13: 3424–3428. doi: 10.1002/pmic.201300271
- Issue published online: 16 DEC 2013
- Article first published online: 4 DEC 2013
- Accepted manuscript online: 31 OCT 2013 02:56AM EST
- Manuscript Accepted: 23 OCT 2013
- Manuscript Revised: 22 AUG 2013
- Manuscript Received: 2 JUL 2013
- German Ministry for Education and Research. Grant Numbers: 0315082, 01EA1303
- European Union. Grant Number: 262055
- Max Planck Society
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Figure S1 Differentiated 3T3-L1 adipocytes after 24 h incubation. (A) 3T3-L1 adipocytes monolayer and (B) 3T3-L1 adipocytes with RAW 264.7 macrophages in the bilayer system.
Figure S2 Validation of molecular effects on the interaction of murine 3T3L1 adipocytes and RAW 264.7 macrophages.
Figure S3 Regression analyses of the duplicates of monolayer and bilayer experiments for adipocytes and macrophages.
Figure S4 VENN diagram of identified proteins (≥2 unique peptides) in differentiated 3T3-L1 adipocytes and RAW 264.7 macrophage cell lines.
Figure S5 Extended analysis focusing on the discrepancy of detected transcripts and proteins.
Figure S6 Numbers of identified phosphopeptides in adipocytes and macrophages.
Table S1: Gene Set Enrichment Analysis: Overview of identified and enriched gene sets in KEGG dataset.
Table S2: Nonlinear HPLC gradient for peptide separation on C18 column.
Table S3: Cut-off limits, empirically determined for diff. 3T3-L1 and RAW 264.7 control experiment, contains 99% of all identified SILAC proteins.
Table S4: Phosphosite analysis results for RAW 264.7 cells in bilayer
Table S5: Phosphosite analysis results for 3T3-L1 cells in bilayer
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