As a service to our authors and readers, this journal provides supporting information supplied by the authors. Such materials are peer reviewed and may be re-organized for online delivery, but are not copy-edited or typeset. Technical support issues arising from supporting information (other than missing files) should be addressed to the authors.


Figure S1 Differentiated 3T3-L1 adipocytes after 24 h incubation. (A) 3T3-L1 adipocytes monolayer and (B) 3T3-L1 adipocytes with RAW 264.7 macrophages in the bilayer system.

Figure S2 Validation of molecular effects on the interaction of murine 3T3L1 adipocytes and RAW 264.7 macrophages.

Figure S3 Regression analyses of the duplicates of monolayer and bilayer experiments for adipocytes and macrophages.

Figure S4 VENN diagram of identified proteins (≥2 unique peptides) in differentiated 3T3-L1 adipocytes and RAW 264.7 macrophage cell lines.

Figure S5 Extended analysis focusing on the discrepancy of detected transcripts and proteins.

Figure S6 Numbers of identified phosphopeptides in adipocytes and macrophages.

Table S1: Gene Set Enrichment Analysis: Overview of identified and enriched gene sets in KEGG dataset.

Table S2: Nonlinear HPLC gradient for peptide separation on C18 column.

Table S3: Cut-off limits, empirically determined for diff. 3T3-L1 and RAW 264.7 control experiment, contains 99% of all identified SILAC proteins.

Table S4: Phosphosite analysis results for RAW 264.7 cells in bilayer

Table S5: Phosphosite analysis results for 3T3-L1 cells in bilayer

pmic7599-sup-0002-SuppMat.rar10515KSupporting Information
pmic7599-sup-0003-SuppMat.doc93KSupporting Information

Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.