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Supplementary figure S1 Comparison of the mass spectrum of Cytochrome C standard solution acquired with ESI (a) and LAESI (b). The electrospray spectra were measured with the IonMax source at a flow rate of 5 ul/min and an electrospray voltage of 4.2 kV.

Supplementary figure S2 Full summed mass spectrum from the CID MS imaging experiment of mouse lung.

Supplementary figure S3 Zoom mass spectrum from the CID MS imaging experiment of mouse lung showing two overlapping fragment ions.

Supplementary figure S3 Full summed spectrum from the IRMPD MS imaging experiment of mouse lung.

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