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pmic7717-sup-0001-FigureS1.eps573KFigure S1: Flowchart of the integrated top-down and bottom-up method for comprehensive characterization of IgGs
pmic7717-sup-0002-FigureS2.eps684KFigure S2: Sensitivity and reproducibility of the RPLC-MS analysis of Fab fragments obtained from adalimumab. A. Dilution curve of intact adalimumab Fab fragments at different concentration. B. Extracted ion chromatograms for three replicated injections with the same sample amount loaded on the column.
pmic7717-sup-0003-FigureS3.eps1272KFigure S3: Coverage achieved with a database search against the accurate sequence of adalimumab with PeaksDB software. The blue bars under the sequence represent unique fragments that were found in the dataset.
pmic7717-sup-0004-FigureS4.eps1104KFigure S4: Sequence coverage achieved for the adalimumab heavy and light chain in subsequent steps of the process. Shown is the accurate sequence of adalimumab, with green highlight showing a successful identification, and red highlights showing mistakes or missing data.
pmic7717-sup-0005-TableS1.xlsx28KTable S1
pmic7717-sup-0006-TableS2.xlsx28KTable S2

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