Label-free LC–MS/MS identification of phosphatidylglycerol-regulated proteins in Synechocystis sp. PCC6803
Article first published online: 14 APR 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Volume 14, Issue 9, pages 1053–1057, May 2014
How to Cite
Talamantes, T., Ughy, B., Domonkos, I., Kis, M., Gombos, Z. and Prokai, L. (2014), Label-free LC–MS/MS identification of phosphatidylglycerol-regulated proteins in Synechocystis sp. PCC6803. Proteomics, 14: 1053–1057. doi: 10.1002/pmic.201300372
- Issue published online: 14 APR 2014
- Article first published online: 14 APR 2014
- Accepted manuscript online: 26 FEB 2014 07:49PM EST
- Manuscript Accepted: 20 FEB 2014
- Manuscript Revised: 4 JAN 2014
- Manuscript Received: 22 AUG 2013
- Welch Foundation. Grant Numbers: BK-0031, OTKA K82052, TAMOP 4.2.2.A-11/1/KONV-2012-0047
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Figure S1. Prefractionation of cellular proteins obtained from the PG-depleted pgsA- mutant of Synechocystis sp. PCC6803 by SDS-PAGE for LC(MS/MS –based proteomics
Figure S2. Schematic illustration of the workflow to identify differentially expressed proteins in pgsA- mutant of Synechocystis sp. PCC6803 by LC(MS/MS-based label-free proteomics using the Progenesis LC-MS software
Figure S2 (cont'd). Typical visualization for inspection and/or validation of results: Probable porin protein (slr1841, predicted) downregulated in the PG-depleted pgsA- mutant of Synechocystis sp. PCC6803.
|pmic7701-sup-0002-TableS1.xlsx||5754K||Supplementary Table S1. Mascot search results.|
|pmic7701-sup-0003-TableS2.xlsx||27K||Supplementary Table S2. PG-regulated peptides using label-free quantification by Progenesis LC-MS (ANOVA with P<0.05) and Mascot database search, requiring at least a twofold change in expression and passing validation by Peptide Prophet, Protein Prophet and at least two unique peptides per protein.|
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