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MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma

Authors

  • Elizabeth Ellen Jones,

    1. Department of Cell and Molecular Pharmacology, MUSC Proteomics Center, Medical University of South Carolina, Charleston, SC, USA
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    • These authors contributed equally to this work.

  • Thomas W. Powers,

    1. Department of Cell and Molecular Pharmacology, MUSC Proteomics Center, Medical University of South Carolina, Charleston, SC, USA
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    • These authors contributed equally to this work.

  • Benjamin A. Neely,

    1. Department of Cell and Molecular Pharmacology, MUSC Proteomics Center, Medical University of South Carolina, Charleston, SC, USA
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  • Lisa H. Cazares,

    1. Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA
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    • Current address: Lisa H. Cazares, United States Army Medical Research Institute of Infectious Diseases, Fort Detrick MD 21702, USA

  • Dean A. Troyer,

    1. Department of Microbiology and Molecular Cell Biology, Eastern Virginia Medical School, Norfolk, VA, USA
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  • Alexander S. Parker,

    1. Departments of Health Sciences Research and Urology, Mayo Clinic Florida, Jacksonville, FL, USA
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  • Richard R. Drake

    Corresponding author
    1. Department of Cell and Molecular Pharmacology, MUSC Proteomics Center, Medical University of South Carolina, Charleston, SC, USA
    • Correspondence: Dr. Richard R. Drake, Department of Cell and Molecular Pharmacology, Medical University of South Carolina, 173 Ashley Avenue, Charleston, SC 29425, USA

      E-mail: draker@musc.edu

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  • Colour Online: See the article online to view Figs. 2 and 4 in colour.

Abstract

Reducing the incidence and mortality rates for clear cell renal cell carcinoma (ccRCC) remains a significant clinical challenge with poor 5-year survival rates. A unique tissue cohort was assembled of matched ccRCC and distal nontumor tissues (n = 20) associated with moderate risk of disease progression, half of these from individuals who progressed to metastatic disease and the other half who remained disease free. These tissues were used for MALDI imaging MS profiling of proteins in the 2–20 kDa range, resulting in a panel of 108 proteins that had potential disease-specific expression patterns. Protein lysates from the same tissues were analyzed by MS/MS, resulting in identification of 56 proteins of less than 20 kDa molecular weight. The same tissues were also used for global lipid profiling analysis by MALDI-FT-ICR MS. From the cumulative protein and lipid expression profile data, a refined panel of 26 proteins and 39 lipid species was identified that could either distinguish tumor from nontumor tissues, or tissues from recurrent disease progressors from nonrecurrent disease individuals. This approach has the potential to not only improve prognostic assessment and enhance postoperative surveillance, but also to inform on the underlying biology of ccRCC progression.

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