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pmic7727-sup-0001-FigureS1.tif8479KFigure S1. M1 and M2 polarized macrophages response to C. albicans. (A) Representative images of C. albicans SC5314 phagocytosis assays by M1 and M2 macrophages at a MOI 1:1. (B) Quantification of phagocytosis of C. albicans yeasts. (C) Candidacidal activity of M1 and M2 macrophages at different co-incubation times. The values in the y axis represent the percentage of C. albicans SC5314 CFU inhibition after the interaction with M1 and M2 macrophages with respect to control yeasts. (D) IL-10, IL-12p40 and TNFα levels of C. albicans-stimulated M1- and M2-polarized macrophages after 3, 6, 12 and 18h of co-incubation.
pmic7727-sup-0002-FigureS2.tif1197KFigure S2. Flow chart for analysis of M1 and M2 macrophages proteome and their response to C. albicans. Workflow of the quantitative 2-D DIGE. Four replicates of M1 and M2 macrophages, control and after 8h of interaction with C. albicans were obtained and labelled with Cy3 and Cy5. Internal standard consisted of a pool of all the protein samples used in the experiment. Differentially expressed proteins determined by 2-D DIGE were excised from the gel and identified by Mass Spectrometry.
pmic7727-sup-0003-FigureS3.tif2466KFigure S3. Functional classification of proteins differentially expressed between M1 and M2 macrophages. Pie chart representation and tables of the Gene Ontology classification of differentially expressed proteins between M1 and M2, as categorized by Genecodis according to their Biological Process (A) and KEGG Pathways (B).

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