These authors contributed equally to this work.
Comprehensive proteomic analysis of a Chinese 2-herb formula (Astragali Radix and Rehmanniae Radix) on mature endothelial cells
Article first published online: 21 AUG 2014
© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Special Issue: Focus on Biomedical Research Trends
Volume 14, Issue 17-18, pages 2089–2103, September 2014
How to Cite
Tam, J. C. W., Ko, C. H., Zhang, C., Wang, H., Lau, C. P., Chan, W. Y., Leung, P. C., Fung, K. P., Zhang, J. F. and Lau, C. B. S. (2014), Comprehensive proteomic analysis of a Chinese 2-herb formula (Astragali Radix and Rehmanniae Radix) on mature endothelial cells. Proteomics, 14: 2089–2103. doi: 10.1002/pmic.201300547
Colour Online: See the article online to view Figs. 2–4 and 6 in colour.
- Issue published online: 8 SEP 2014
- Article first published online: 21 AUG 2014
- Accepted manuscript online: 12 JUL 2014 06:21AM EST
- Manuscript Accepted: 7 JUL 2014
- Manuscript Revised: 8 JUN 2014
- Manuscript Received: 10 DEC 2013
- Chinese Medicine Research and Further Development. Grant Number: AoE/B-10/01
- Astragali Radix;
- Endothelial cells;
- Rehmanniae Radix;
- Wound healing
Endothelial cells are crucially involved in wound healing angiogenesis, restoring blood flow to wound tissues. Our previous study demonstrated that the Chinese 2-herb formula (NF3) possesses significant wound healing effect in diabetic foot ulcer rats with promising in vitro proangiogenic effects on human umbilical vein endothelial cells (HUVEC). Here, we present the comparative global proteome analysis of NF3-treated HUVEC in static or scratch conditions, screening the comprehensive molecular targets in governing the proangiogenic response in wound healing. Our results suggest plasminogen activator inhibitor-1, specifically down-regulated in static condition and Annexin A1 and Annexin A2, up-regulated in scratch condition, as principal proteins responsible for the proangiogenesis in wound healing. We also identified a panel of cytoskeleton regulatory proteins in static and scratch condition, mediating the migratory behavior of NF3-treated HUVEC. The key proteins in static state include myosin regulatory light polypeptide 9, SPAST, tropomyosin (TPM)2, and Vimentin while that in scratch state contained prelamin-A/C, TPM1, TPM2, and Vimentin. In addition, NF3 was shown to regulate transcription and translation, cell–cell interaction, and ROS defense in HUVEC. Proliferation and migration assays further confirmed the identified principal proteins plasminogen activator inhibitor-1 and Annexin A2 which are responsible for NF3-induced proangiogenesis of HUVEC in wound healing. This is the first study on the global proteome expression of NF3-treated HUVEC with the identification of the differences at the molecular level, between static and scratch conditions involved in wound healing angiogenesis.