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FilenameFormatSizeDescription
pmic7805-sup-0001-FigureS1.pptx140KKlein et al. Supplementary figure 1
pmic7805-sup-0002-FigureS2.pptx463KKlein et al. Supplementary figure 2. Principal component analysis (PCA) of mass spectra from traumatized (tm), trauma adjacent (tam) and healthy (hm) muscle regions. A) PCA analysis of MALDI-Imaging spectra of two tissue sections containing regions of trauma (tm), trauma adjacent (tam), and healthy (hm) muscle tissue regions. Top row: the spectra in the first 3 principal components (from left to right: PC1–3, PC1–2, PC1–3, PC2–3), tm spectra in red, tam spectra in green, hm spectra in blue. Middle row: PCA loadings. Bottom row: variance explanation by first 10 principal components. B) Intensity distribution of the first principal component PC1 for the considered tissue sections which clearly discriminates trauma (tm), low intensity of PC1, trauma adjacent (tam), moderate intensity of PC1, and healthy (hm) muscles, high intensity of PC1. C) HE staining of the healthy and the traumatized soleus muscle is shown in the bottom right subplot and region of interest are indicated by the red ( = tm), black ( = tam) and green ( = hm) frame
pmic7805-sup-0003-FigureS3.pptx503KKlein et al. Supplementary figure 3.Spatial distribution of characteristic m/z values for the traumatized, trauma adjacent and healthy muscle regions of all biological. Ion density distribution of (A) m/z value 976 (identified as Acts) and (B) m/z value 903 (identified as Ca3) by using supervise approach (ClinProTools) and unsupervise approach (SCilS Lab). (C) Shown are the HE staining of the healthy and the injured soleus muscle in the below subplot and region of interest are indicated by the red ( = tm), black ( = tam) and green ( = hm) frame.
pmic7805-sup-0004-TableS1.xlsx196KSupplementray table 1: ClinProt peak statistic to discriminate traumatized tissue and trauma adjacent muscle tissue
pmic7805-sup-0005-TableS2.xlsx23KSupplementary table 2: Data evaluation by SCiLS (grey rows). M/z-values found to be co-localized with trauma tissue. M/z values which were identified by nanoLC mass spectrometry are shown in the green rows. The mass differences between the observed mass (Imaging) and identified mass (LC-MS/MS) are shown in the D[Da] row.
pmic7805-sup-0006-TableS3.xlsx19KSupplementary table 3: Data evaluation by SCiLS (grey rows). M/z-values found to be co-localized with healthy and trauma adjacent tissue. M/z values which were identified by nanoLC mass spectrometry are shown in the green rows. The mass differences between the observed mass (Imaging) and identified mass (LC-MS/MS) are shown in the D[Da] row.
pmic7805-sup-0007-TableS4.xlsx310KSupplemantary table 4: Identified proteins by corresponding bottom up LC-MS/MS approach with adjacent tissue sections.
pmic7805-sup-0008-TableS5.xlsx18KSupplementary table 5: Identified MALDI-IMS m/z values by LC-MS/MS. The mass differences between the observed mass (Imaging) and identified mass are shown in the D[Da] row.

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