Extracellular Ser/Thr/Tyr phosphorylated proteins of Pseudomonas aeruginosa PA14 strain

Authors

  • Tassadit Ouidir,

    1. Polymers, Biopolymers, Surfaces Laboratory, CNRS, Mont-Saint-Aignan, France
    2. Normandie University, UR, Mont-Saint-Aignan, France
    3. PISSARO Proteomic Platform, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont Saint Aignan, France
    Search for more papers by this author
  • Frédérique Jarnier,

    1. Normandie University, UR, Mont-Saint-Aignan, France
    2. PISSARO Proteomic Platform, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont Saint Aignan, France
    Search for more papers by this author
  • Pascal Cosette,

    1. Polymers, Biopolymers, Surfaces Laboratory, CNRS, Mont-Saint-Aignan, France
    2. Normandie University, UR, Mont-Saint-Aignan, France
    3. PISSARO Proteomic Platform, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont Saint Aignan, France
    Search for more papers by this author
  • Thierry Jouenne,

    1. Polymers, Biopolymers, Surfaces Laboratory, CNRS, Mont-Saint-Aignan, France
    2. Normandie University, UR, Mont-Saint-Aignan, France
    3. PISSARO Proteomic Platform, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont Saint Aignan, France
    Search for more papers by this author
  • Julie Hardouin

    Corresponding author
    1. Polymers, Biopolymers, Surfaces Laboratory, CNRS, Mont-Saint-Aignan, France
    2. Normandie University, UR, Mont-Saint-Aignan, France
    3. PISSARO Proteomic Platform, Institute for Research and Innovation in Biomedicine, University of Rouen, Mont Saint Aignan, France
    • Correspondence: Dr. Julie Hardouin, Laboratoire Polymères, Biopolymères, Surfaces, UMR CNRS 6270, Université de Rouen, 76821 Mont-Saint-Aignan cedex, France

      E-mail: julie.hardouin@univ-rouen.fr

    Search for more papers by this author

Abstract

Protein phosphorylation on serine, threonine, and tyrosine is known to be involved in a wide variety of cellular processes, signal transduction, and bacterial virulence. We characterized, for the first time, the extracellular phosphoproteins of the Pseudomonas aeruginosa PA14 strain. We identified 28 phosphoproteins (59 phosphosites) including enzymes, with various phosphorylation sites, known as potent secreted virulence factors in P. aeruginosa. The high phosphorylation level of these virulence factors might reflect a relationship between Ser/Thr/Tyr phosphorylation and virulence.

Ancillary