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Research Article

Proteomic analysis of a compatible interaction between sugarcane and Sporisorium scitamineum

Authors

  • Leonard Barnabas,

    1. Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India
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  • N. M. R. Ashwin,

    1. Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India
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  • K. Kaverinathan,

    1. Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India
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  • Anna Rita Trentin,

    1. Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy
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  • Micaela Pivato,

    1. Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy
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  • A. Ramesh Sundar,

    Corresponding author
    1. Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India

    • Correspondence: Dr. A. Ramesh Sundar, Plant Pathology Section, Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Coimbatore 641 007, Tamil Nadu, India

      E-mail: rameshsundar_sbi@yahoo.co.in

      Fax: +91-0422-2472923

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  • P. Malathi,

    1. Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India
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  • R. Viswanathan,

    1. Division of Crop Protection, ICAR-Sugarcane Breeding Institute, Indian Council of Agricultural Research, Coimbatore, India
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  • O. B. Rosana,

    1. Bioinformatics Center, ICAR-Indian Institute of Spices Research, Kozhikode, India
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  • K. Neethukrishna,

    1. Bioinformatics Center, ICAR-Indian Institute of Spices Research, Kozhikode, India
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  • Paolo Carletti,

    1. Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy
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  • Giorgio Arrigoni,

    1. Proteomics Center of Padova University, Padova, Italy
    2. Department of Biomedical Sciences, University of Padova, Padova, Italy
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  • Antonio Masi,

    1. Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padova, Padova, Italy
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  • Ganesh Kumar Agrawal,

    1. Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal
    2. GRADE (Global Research Arch for Developing Education) Academy Private Limited, Birgunj, Nepal
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  • Randeep Rakwal

    1. Research Laboratory for Biotechnology and Biochemistry (RLABB), Kathmandu, Nepal
    2. GRADE (Global Research Arch for Developing Education) Academy Private Limited, Birgunj, Nepal
    3. Tsukuba International Academy for Sport Studies (TIAS) and Faculty of Health and Sport Sciences, University of Tsukuba, Ibaraki, Japan
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  • Colour Online: See the article online to view Figs. 2, 3 and 4 in colour.

Abstract

Smut caused by Sporisorium scitamineum is one of the important diseases of sugarcane with global significance. Despite the intriguing nature of sugarcane, S. scitamineum interaction, several pertinent aspects remain unexplored. This study investigates the proteome level alterations occurring in the meristem of a S. scitamineum infected susceptible sugarcane cultivar at whip emergence stage. Differentially abundant proteins were identified by 2DE coupled with MALDI-TOF/TOF-MS. Comprehensively, 53 sugarcane proteins identified were related to defence, stress, metabolism, protein folding, energy, and cell division; in addition, a putative effector of S. scitamineum, chorismate mutase, was identified. Transcript expression vis-à-vis the activity of phenylalanine ammonia lyase was relatively higher in the infected meristem. Abundance of seven candidate proteins in 2D gel profiles was in correlation with its corresponding transcript expression levels as validated by qRT-PCR. Furthermore, this study has opened up new perspectives on the interaction between sugarcane and S. scitamineum.

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Article Information

DOI

10.1002/pmic.201500245

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© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

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Keywords

  • Compatible interaction;
  • Smut;
  • Sporisorium scitamineum;
  • Sugarcane;
  • Plant proteomics

Publication History

  • Issue online:
  • Version of record online:
  • Accepted manuscript online:
  • Manuscript Accepted:
  • Manuscript Revised:
  • Manuscript Received:

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pmic12260-sup-0002-FigS2A.tif981KSupporting Information
pmic12260-sup-0003-FigS2B.tif671KSupporting Information
pmic12260-sup-0004-TableS1.doc63KSupporting Information
pmic12260-sup-0005-TableS2.xls38KSupporting Information

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  • Number of times cited: 5
  1. 1Leila P. Peters, Giselle Carvalho, Milca B. Vilhena, Silvana Creste, Ricardo A. Azevedo, Claudia B. Monteiro-Vitorello, Functional analysis of oxidative burst in sugarcane smut-resistant and -susceptible genotypes, Planta, 2017, 245, 4, 749CrossRef
  2. 2Leonard Barnabas, N.M.R. Ashwin, Kalimuthu Kaverinathan, Anna Rita Trentin, Micaela Pivato, Amalraj Ramesh Sundar, Palaniyandi Malathi, Rasappa Viswanathan, Paolo Carletti, Giorgio Arrigoni, Antonio Masi, Ganesh Kumar Agrawal, Randeep Rakwal, In vitro secretomic analysis identifies putative pathogenicity-related proteins of Sporisorium scitamineum – The sugarcane smut fungus, Fungal Biology, 2017, 121, 3, 199CrossRef
  3. 3Leonard Barnabas, N. M. R. Ashwin, A. Ramesh Sundar, P. Malathi, R. Viswanathan, Putative orthologs of Ustilago maydis effectors screened from the genome of sugarcane smut fungus - Sporisorium scitamineum , Australasian Plant Pathology, 2017CrossRef
  4. 4Elena Sánchez-Elordi, František Baluška, Clara Echevarría, Carlos Vicente, M. Estrella Legaz, Defence sugarcane glycoproteins disorganize microtubules and prevent nuclear polarization and germination of Sporisorium scitamineum teliospores, Journal of Plant Physiology, 2016, 200, 111CrossRef
  5. 5Patricia D. C. Schaker, Alessandra C. Palhares, Lucas M. Taniguti, Leila P. Peters, Silvana Creste, Karen S. Aitken, Marie-Anne Van Sluys, João P. Kitajima, Maria L. C. Vieira, Claudia B. Monteiro-Vitorello, Jason E Stajich, RNAseq Transcriptional Profiling following Whip Development in Sugarcane Smut Disease, PLOS ONE, 2016, 11, 9, e0162237CrossRef