Immobilization of glucose oxidase on nylon membranes and its application in a flow-through glucose reactor
Article first published online: 10 MAR 2003
Copyright © 1991 John Wiley & Sons, Inc.
Journal of Polymer Science Part A: Polymer Chemistry
Volume 29, Issue 2, pages 275–279, February 1991
How to Cite
Da Silva, M. A., Gil, M. H., Redinha, J. S., Brett, A. M. O. and Pereira, J. L. C. (1991), Immobilization of glucose oxidase on nylon membranes and its application in a flow-through glucose reactor. J. Polym. Sci. A Polym. Chem., 29: 275–279. doi: 10.1002/pola.1991.080290216
- Issue published online: 10 MAR 2003
- Article first published online: 10 MAR 2003
- Manuscript Accepted: 16 JUL 1990
- Manuscript Received: 2 MAR 1990
The immobilization of glucose oxidase on hydrolyzed nylon-6,6 was studied. Various spacers were introduced on the support before the coupling of the enzyme. Best results were obtained when the membrane was covered with denatured bovine serum albumin (BSA) before spacer coupling and immobilization of glucose oxidase (GOD). The influence of various factors (pH, ionic strength, etc.) on the activity of the free and immobilized enzyme was investigated. It was found that the behavior of the fixed glucose oxidase and the free enzyme is very similar. The covalently immobilized enzyme had a lifetime of around 2 months (50% of initial activity).