Synthesis and characterization of fluorescent copolymer containing rare earth metal complex and its interaction with DNA

Authors

  • Xiao-Xi Hu,

    1. Faculty of Materials Science and Engineering, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, People's Republic of China
    2. Division of Spine Surgery, Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, China
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  • Peng-Hui Li,

    1. Faculty of Materials Science and Engineering, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, People's Republic of China
    2. Division of Spine Surgery, Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, China
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  • Kelvin W. K. Yeung,

    1. Division of Spine Surgery, Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, China
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  • Paul K. Chu,

    1. Department of Physics & Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China
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  • Shui-Lin Wu,

    1. Faculty of Materials Science and Engineering, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, People's Republic of China
    2. Department of Physics & Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China
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  • Zu-Shun Xu

    Corresponding author
    1. Faculty of Materials Science and Engineering, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, People's Republic of China
    2. Division of Spine Surgery, Department of Orthopaedics and Traumatology, The University of Hong Kong, Pokfulam, Hong Kong, China
    3. Department of Physics & Materials Science, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong, China
    • Faculty of Materials Science and Engineering, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei University, Wuhan 430062, People's Republic of China
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Abstract

A complex of Eu3+, acrylic acid (AA), and 1, 10-phenanthroline (Phen) was synthesized. The structure and fluorescence of Eu(AA)3Phen was characterized with elemental analysis, FTIR, 1H NMR, and fluorescence spectroscopy. A novel copolymer containing rare earth complex, poly(PEGMA-co-NIPAm-co-Eu(AA)3Phen) (PPNEu), was prepared by free radical copolymerization in methanol with azodiisobutyronitrile as initiator. 1H NMR, fluorescence spectroscopy, UV-vis spectroscopy, and TEM were used to characterize this copolymer. The interaction of PPNEu with deoxyribonucleic acid (DNA) was studied by fluorescence spectroscopy, UV-vis spectroscopy and agarose gel electrophoresis. The results of fluorescence, UV-vis absorption, and agarose electrophoresis indicated that the PPNEu could interact with DNA in an electrostatic bonding mode. The TEM observation showed that the PPNEu could form spherical micelles in water solution small than 100 nm; the efficient complexation of PPNEu with DNA occurred. These results suggested the potential of the PPNEu as gene detective reagent and gene delivery carrier. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010

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