Poly(L-glutamic acid) grafted with oligo(2-(2-(2-methoxyethoxy)ethoxy)ethyl methacrylate): Thermal phase transition, secondary structure, and self-assembly

Authors

  • Jianxun Ding,

    1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
    2. Graduate University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China
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  • Chunsheng Xiao,

    1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
    2. Graduate University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China
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  • Li Zhao,

    1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
    2. Graduate University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China
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  • Yilong Cheng,

    1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
    2. Graduate University of Chinese Academy of Sciences, Beijing 100039, People's Republic of China
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  • Lili Ma,

    1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
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  • Zhaohui Tang,

    1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
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  • Xiuli Zhuang,

    1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
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  • Xuesi Chen

    Corresponding author
    1. Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
    • Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China
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Abstract

Thermoresponsive and pH-responsive graft copolymers, poly(L-glutamate)-g-oligo(2-(2-(2-methoxyethoxy)ethoxy)ethyl methacrylate) and poly(L-glutamic acid-co-(L-glutamate-g-oligo(2-(2-(2-methoxyethoxy)ethoxy)ethyl methacrylate))), were synthesized by ring-opening polymerization (ROP) of N-carboxyanhydride (NCA) monomers and subsequent atom transfer radical polymerization of 2-(2-(2-methoxyethoxy)ethoxy)ethyl methacrylate. The thermoresponsiveness of graft copolymers could be tuned by the molecular weight of oligo(2-(2-(2-methoxyethoxy)ethoxy)ethyl methacrylate) (OMEO3MA), composition of poly(L-glutamic acid) (PLGA) backbone and pH of the aqueous solution. The α-helical contents of graft copolymers could be influenced by OMEO3MA length and pH of the aqueous solution. In addition, the graft copolymers exhibited tunable self-assembly behavior. The hydrodynamic radius (Rh) and critical micellization concentration values of micelles were relevant to the length of OMEO3MA and the composition of biodegradable PLGA backbone. The Rh could also be adjusted by the temperature and pH values. Lastly, in vitro methyl thiazolyl tetrazolium (MTT) assay revealed that the graft copolymers were biocompatible to HeLa cells. Therefore, with good biocompatibility, well-defined secondary structure, and mono-, dual-responsiveness, these graft copolymers are promising stimuli-responsive materials for biomedical applications. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011

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