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Magnetic poly(N-propargylacrylamide) microspheres: Preparation by precipitation polymerization and use in model click reactions

Authors

  • Hana Macková,

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic
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  • Vladimír Proks,

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic
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  • Daniel Horák,

    Corresponding author
    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic
    • Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic
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  • Jan Kučka,

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic
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  • Miroslava Trchová

    1. Institute of Macromolecular Chemistry, Academy of Sciences of the Czech Republic, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic
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Abstract

Magnetic poly(N-propargylacrylamide) (PPRAAm) microspheres were prepared by the precipitation polymerization of N-propargylacrylamide (PRAAm) in a toluene/propan-2-ol medium in the presence of magnetic nanoparticles (oleic acid-coated Fe3O4). The effects of several polymerization parameters, including the polarity of the medium, polymerization temperature, the concentration of monomer, and the amount of magnetite (Fe3O4) in the polymerization feed, were examined. The microspheres were characterized in terms of their morphology, size, particle-size distribution, and iron content using transmission and scanning electron microscopies (TEM and SEM) and atomic absorption spectroscopy (AAS). A medium polarity was identified in which magnetic particles with a narrow size distribution were formed. As expected, oleic acid-coated Fe3O4 nanoparticles contributed to the stabilization of the polymerized magnetic microspheres. Alkyne groups in magnetic PPRAAm microspheres were detected by infrared spectroscopy. Magnetic PPRAAm microspheres were successfully used as the anchor to enable a “click” reaction with an azido-end-functionalized model peptide (radiolabeled azidopentanoyl-GGGRGDSGGGY(125I)-NH2) and 4-azidophenylalanine using a Cu(I)-catalyzed 1,3-dipolar azide-alkyne cycloaddition reaction in water. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011.

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