Water-soluble polymer-grafted platinum nanoparticles for the subsequent binding of enzymes. synthesis and SANS

Authors

  • François Gal,

    1. Laboratoire Léon Brillouin (CEA/DSM/IRAMIS/LLB-CNRS UMR 0012), Bâtiment 563, CEA/Saclay, 91191 Gif-sur-Yvette, France
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  • Henri Perez,

    1. Laboratoire Francis Perrin (CEA/DSM/IRAMIS/SPAM-CNRS URA 2453), Bâtiment 522, CEA/Saclay, 91191 Gif-sur-Yvette, France
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  • Vincent Noel,

    1. Interfaces, Traitement, Organisation et Dynamiques des Systèmes (ITODYS), University Paris 7-Paris Diderot, UMR CNRS 7086, 15 rue Jean de Baïf, 75205 Paris Cedex 13, France
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  • Geraldine Carrot

    Corresponding author
    1. Laboratoire Léon Brillouin (CEA/DSM/IRAMIS/LLB-CNRS UMR 0012), Bâtiment 563, CEA/Saclay, 91191 Gif-sur-Yvette, France
    • Laboratoire Léon Brillouin (CEA/DSM/IRAMIS/LLB-CNRS UMR 0012), Bâtiment 563, CEA/Saclay, 91191 Gif-sur-Yvette, France
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Abstract

Functionalized platinum nanoparticles (PtNPs) possess catalytic properties towards H202 oxidation, which are of great interest for the elaboration of electrochemical biosensors. To improve the understanding of phenomena involved in such systems, we designed platinum-polymer-enzyme model nanostructures according to a bottom–up approach. These structures have been elaborated from elementary building units based on polymer-grafted PtNPs obtained from surface initiated-atom transfer radical polymerization. This paper describes the polymerization of ter-butyl methacrylate from PtNPs and its subsequent hydrolysis to obtain a water-soluble corona, followed by an activated ester modification to introduce an enzyme (glucose oxidase). The structure of the objects, the molecular weight and the grafting density of the polymer chains were principally elucidated by small angle neutron scattering (SANS). After the grafting of the enzyme, the final hybrid structures were characterized by both microscopy and SANS to attest for the covalent grafting of the enzyme. Composition and enzyme activity of the nanohybrid objects, have also been determined by UV spectroscopy. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012

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