Cover Image, Volume 49, Issue 14


original image

Polynucleotide translocation is a process in which biomolecules, like DNA or RNA, are electrophoretically driven through a narrow pore and their passage is monitored by the change in the ionic current through the pore. Interest in the process has moved from purely biological to technological, since it offers the chance for ultra-fast, low-cost sequencing of DNA–so-called ‘nanopore sequencing’. On page 985 of this issue, Maria Fyta and colleagues review the progress in the field of polynucleotide translocation, across experimental, theoretical and computational approaches. With this background in mind, they also discuss the challenges remaining to the use of translocation for DNA sequencing.