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Keywords:

  • biopolymers;
  • di(ethylene glycol) mono vinyl ether (EO2);
  • plasma enhanced chemical vapor deposition (PECVD);
  • polymerization;
  • surface plasmon enhanced fluorescence spectroscopy (SPEFS)

Abstract

Thumbnail image of graphical abstract

The immobilization of biotin-streptavidin within an antifouling pp-EO2 plasma polymer.

Summary: We report on the development of a multilayer system, which allows for specific DNA binding reactions to occur at the surface, but at the same time shows antifouling properties, thus minimizing the nonspecific adsorption of proteins (BSA). The synthesis involves the combination of plasma polymerization and streptavidin-biotin assembly. DNA sensing is achieved using SPFS. Antifouling properties are tailored by controlling the film thickness, together with the effects of the swelling in buffer solution and by the specific surface chemical design. Preliminary hybridization experiments show promising results.