Assessment of Damage of Glutathione by Glow Discharge Plasma at the Gas–Solution Interface through Raman Spectroscopy

Authors

  • Zhigang Ke,

    1. Key Laboratory of Ion Beam Bio-engineering, Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
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  • Zengliang Yu,

    1. Key Laboratory of Ion Beam Bio-engineering, Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
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  • Qing Huang

    Corresponding author
    1. Key Laboratory of Ion Beam Bio-engineering, Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
    2. University of Science and Technology of China, Hefei 230026, China
    • Key Laboratory of Ion Beam Bio-engineering, Institute of Technical Biology and Agriculture Engineering, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China
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Abstract

Although non-thermal discharge plasma is more and more applied in biological field, the molecular mechanisms of plasma acting on biomolecules are still unclear, which are indispensable for understanding the plasma-induced biological effects. In this work, discharge plasma at the gas–solution interface was employed to irradiate on reduced glutathione (GSH), the most abundant low molecular weight thiol-containing antioxidant in cells. Through Raman spectroscopy, both the irreversible damage and reversible conversion of GSH to oxidized glutathione (GSSG) were monitored in a non-destructive fashion, so that the reaction kinetic processes through multi-pathways could be evaluated quickly and quantitatively. Based on the experimental data, the reaction mechanism was discussed.

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