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Thromboelastography (TEG) Cups and Pins with Different PECVD Coatings: Effect on the Coagulation Cascade in Platelet-poor Blood Plasma

Authors

  • Angel Contreras-García,

    1. Groupe de Physique et Technologie des Couches Minces (GCM), Department of Engineering Physics, École Polytechnique de Montréal, Montréal, QC, Canada
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  • Yahye Merhi,

    1. Montreal Heart Institute, Université de Montréal, Montréal, QC H1T 1C8, Canada
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  • Juan-Carlos Ruiz,

    1. Groupe de Physique et Technologie des Couches Minces (GCM), Department of Engineering Physics, École Polytechnique de Montréal, Montréal, QC, Canada
    Current affiliation:
    1. Institut für Mikrotechnik Mainz, Mainz, Germany
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  • Michael R. Wertheimer,

    1. Groupe de Physique et Technologie des Couches Minces (GCM), Department of Engineering Physics, École Polytechnique de Montréal, Montréal, QC, Canada
    2. Institute of Biomedical Engineering, École Polytechnique de Montréal, Montréal, QC H3C 3A7, Canada
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  • Caroline D. Hoemann

    Corresponding author
    1. Department of Chemical Engineering, École Polytechnique de Montréal, Montréal, QC H3C 3A7, Canada
    • Institute of Biomedical Engineering, École Polytechnique de Montréal, Montréal, QC H3C 3A7, Canada
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E-mail: caroline.hoemann@polymtl.ca

Abstract

Thromboelastography uses cups and pins made of Cyrolite® plastic to analyze the rate of fibrin clot formation in blood samples. In this study, TEG cups and pins were modified by 4 distinct coating types using plasma-enhanced chemical vapor deposition (PECVD): carboxylated, amine-rich, hydrophobic, SiO2, and analyzed for surface chemistry and wettability. We tested the hypothesis that the coagulation kinetics of recalcified citrated blood plasma is controlled by surface chemistry, in the absence of clot activator. Only carboxylated surfaces became negatively charged upon wetting, and accelerated clot formation in a highly reproducible manner, whereas Cyrolite® and the other coatings had delayed and unpredictable clotting times. These data are consistent with a model whereby carboxylated surfaces selectively adsorb and activate factor XII while repelling other more abundant anionic blood proteins, resulting in reproducible clot kinetics.ppap201300027-gra-0001

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