Nano-Ruby: A Promising Fluorescent Probe for Background-Free Cellular Imaging (pages 506–513)
Andrew M. Edmonds, Mushtaq A. Sobhan, Varun K. A. Sreenivasan, Ekaterina A. Grebenik, James R. Rabeau, Ewa M. Goldys and Andrei V. Zvyagin
Article first published online: 27 FEB 2013 | DOI: 10.1002/ppsc.201200112
Bioprobes based on fluorescent ruby nanoparticles that are suitable for ultrasensitive imaging are produced by femtosecond laser ablation of bulk ruby. A stable aqueous/buffer colloid, permitting facile conjugation to proteins, arises and the nanoparticles are photostable, with long lifetime emission. With time-gating complete suppression of cell autofluorescence and exogenous fluorophores is observed.