Research Article

Peptidomic analysis of rat urine using capillary electrophoresis coupled to mass spectrometry

  1. Moritz Frommberger1,†,
  2. Petra Zürbig2,†,
  3. Justyna Jantos3,
  4. Thomas Krahn4,
  5. Harald Mischak2,
  6. Andreas Pich5,
  7. Ingo Just5,
  8. Philippe Schmitt-Kopplin1,
  9. Eric Schiffer Dr.2,*

Article first published online: 8 JUN 2007

DOI: 10.1002/prca.200700195

Issue

PROTEOMICS - Clinical Applications

PROTEOMICS - Clinical Applications

Volume 1, Issue 7, pages 650–660, No. 7 July 2007

Additional Information

How to Cite

Frommberger, M., Zürbig, P., Jantos, J., Krahn, T., Mischak, H., Pich, A., Just, I., Schmitt-Kopplin, P. and Schiffer, E. (2007), Peptidomic analysis of rat urine using capillary electrophoresis coupled to mass spectrometry. PROTEOMICS - Clinical Applications, 1: 650–660. doi: 10.1002/prca.200700195

Author Information

  1. 1

    Institute of Ecological Chemistry, GSF - National Research Center for Environment and Health, Neuherberg, Germany

  2. 2

    Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany

  3. 3

    University of Applied Sciences of Oldenburg/Ostfriesland/Wilhelmshaven, Faculty of Science and Technology, Emden, Germany

  4. 4

    Bayer HealthCare AG, Elberfeld, Germany

  5. 5

    Hannover Medical School, Department of Toxicology, Hannover, Germany

  1. †

    These authors contributed equally to this work.

*Mosaiques diagnostics & therapeutics AG, Mellendorfer Str. 7–9, 30625 Hannover, Germany : Fax: +49-511-554744-31

Publication History

  1. Issue published online: 28 JUN 2007
  2. Article first published online: 8 JUN 2007
  3. Manuscript Received: 21 FEB 2007

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Keywords:

  • Biomarker;
  • CE-MS;
  • Rat;
  • Urine

Abstract

We have established and validated a protocol for the peptidomic analysis of rat urine using CE coupled to MS (CE-MS). In the first experiments, the reproducibility of the CE-MS set-up and of the established preparation procedure were assessed. To establish a first rat urinary peptidome map, samples were also analyzed using CE-FT-ICR. The subsequent analysis of independent samples from two different strains (WISTAR and CD) indicated strain-specific differences, which were validated in a blinded assessment. MS/MS revealed the presence of specific fragments from well-known urinary rat peptides, such as collagens, alpha-1-antitrypsin, and serum albumin. The CE-MS-based peptidomics platform may provide novel insights into body fluids of animal models, such as rat or mice. Together with peptide identification, the technology appears to be an excellent, complimentary, and non-invasive tool to analyze toxicological or other (patho)physiological effects of pharmaceutical compounds in animal models.

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