Research Article

Influence of variations in sample handling on SELDI-TOF MS serum protein profiles for colorectal cancer

  1. Judith Y. M. N. Engwegen1,*,
  2. Marieke Alberts1,
  3. Jaco C. Knol2,
  4. Connie R. Jimenez2,
  5. Annekatrien C. T. M. Depla3,
  6. Henriëtte Tuynman3,
  7. Henk A. van Heukelem3,
  8. Pleun Snel3,
  9. Marianne E. Smits4,
  10. Annemieke Cats4,
  11. Jan H. M. Schellens5,6,
  12. Jos H. Beijnen1,6

Article first published online: 12 JUN 2008

DOI: 10.1002/prca.200780068

Issue

PROTEOMICS - Clinical Applications

PROTEOMICS - Clinical Applications

Special Issue: CARDIOVASCULAR PROTEOMICS

Volume 2, Issue 6, pages 936–945, No. 6 June 2008

Additional Information

How to Cite

Engwegen, J. Y. M. N., Alberts, M., Knol, J. C., Jimenez, C. R., Depla, A. C. T. M., Tuynman, H., van Heukelem, H. A., Snel, P., Smits, M. E., Cats, A., Schellens, J. H. M. and Beijnen, J. H. (2008), Influence of variations in sample handling on SELDI-TOF MS serum protein profiles for colorectal cancer. Prot. Clin. Appl., 2: 936–945. doi: 10.1002/prca.200780068

Author Information

  1. 1

    Department of Pharmacy & Pharmacology, The Netherlands Cancer Institute/Slotervaart Hospital, Amsterdam, The Netherlands

  2. 2

    OncoProteomics Laboratory, VUmc Cancer Centre, VU University Medical Center, Amsterdam, The Netherlands

  3. 3

    Department of Gastroenterology and Hepatology, Slotervaart Hospital, Amsterdam, The Netherlands

  4. 4

    Department of Gastroenterology and Hepatology, Antoni van Leeuwenhoek Hospital/ The Netherlands Cancer Institute, Amsterdam, The Netherlands

  5. 5

    Department of Medical Oncology, The Netherlands Cancer Institute/Antoni van Leeuwenhoek Hospital, Amsterdam, The Netherlands

  6. 6

    Utrecht University, Faculty of Science, Department of Pharmaceutical Sciences, Division of Biomedical Analysis, Utrecht, The Netherlands

*Atrium Medisch Centrum Parkstad, Department of Pharmacy, P.O. Box 4446, 6401 CX Heerlen, The Netherlands Fax: +31-45-5767279

Publication History

  1. Issue published online: 12 JUN 2008
  2. Article first published online: 12 JUN 2008
  3. Manuscript Received: 20 SEP 2007

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Keywords:

  • Biomarkers;
  • Colorectal cancer;
  • Sample handling;
  • SELDI-TOF MS

Abstract

Sample handling can have a profound effect on serum protein profiles, challenging results obtained with archived sera under non-standardized sample collection. Here, we evaluate the influence of variations in sample handling on previous serum protein profiles for colorectal cancer (CRC) (Engwegen et al.,. World J. Gastroenterol. 2006, 12, 1536–1544). Sera were prospectively obtained from individuals with an indication for colonoscopy (n = 150: 65 controls, 52 adenomatous polyps, 29 CRC, 4 unknown), as well as from normal volunteers (n = 8). Protein profiles were acquired by SELDI-TOF MS on CM10 chips at pH 5. We assessed the influence of storage temperature, type of collection tube, coagulation temperature and freeze-thaw cycles on the serum protein profile. Several peptides occurred only in samples stored at –20°C, indicating proteolytic degradation during storage. One was a previous CRC biomarker candidate, an N-terminal albumin fragment (m/z 3087), and two others complement C3f and a fragment thereof (m/z 2022 and 1863). Overall differences in protein profiles were also seen for different collection tubes, coagulation temperature and freeze-thaw cycles. However, three of five of our previously defined CRC biomarker candidates are stable to variations in the sample handling protocol, justifying their further validation in prospective studies.

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