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Technical Brief
A method of HIV-1 inactivation compatible with antibody-based depletion of abundant proteins from plasma
Article first published online: 6 MAY 2008
DOI: 10.1002/prca.200780086
Copyright © 2008 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Issue
PROTEOMICS - Clinical Applications
Special Issue: CARDIOVASCULAR PROTEOMICS
Volume 2, Issue 6, pages 904–907, No. 6 June 2008
Additional Information
How to Cite
Bosley, A., Marshall, H. N., Badralmaa, Y. and Natarajan, V. (2008), A method of HIV-1 inactivation compatible with antibody-based depletion of abundant proteins from plasma. Prot. Clin. Appl., 2: 904–907. doi: 10.1002/prca.200780086
Publication History
- Issue published online: 12 JUN 2008
- Article first published online: 6 MAY 2008
- Manuscript Received: 16 OCT 2007
Funded by
- National Institute of Allergy and Infectious Diseases
- National Cancer Institute, National Institutes of Health. Grant Number: NO1-CO-12400
Keywords:
- HIV-1;
- Plasma depletion;
- Plasma proteomics
Abstract
We have examined several methods, including heat treatment and treatment with detergents, to inactivate HIV-1 present in plasma to be depleted of abundant proteins utilizing an antibody-based technology. Treatment with Triton X-100 was not compatible with abundant protein depletion with an antibody column and heat treatment alters the composition of the plasma proteome. However, treatment with 1.2% N-octylglucoside for 5 min completely inhibited HIV-1 infectivity. The detergent was easily removed through buffer exchange, and this treatment had no discernable effect on protein depletion.