Research Article

Stable and unstable angina: Identifying novel markers on circulating leukocytes

  1. Angus Brown1,
  2. Jo-Dee Lattimore2,3,
  3. Michele McGrady2,
  4. David Sullivan3,4,
  5. Wayne Dyer5,6,
  6. Filip Braet7,
  7. Cristobal dos Remedios Professor1,*

Article first published online: 11 DEC 2007

DOI: 10.1002/prca.200780090

Issue

PROTEOMICS - Clinical Applications

PROTEOMICS - Clinical Applications

Volume 2, Issue 1, pages 90–98, No. 1 January 2008

Additional Information

How to Cite

Brown, A., Lattimore, J.-D., McGrady, M., Sullivan, D., Dyer, W., Braet, F. and dos Remedios, C. (2008), Stable and unstable angina: Identifying novel markers on circulating leukocytes. Prot. Clin. Appl., 2: 90–98. doi: 10.1002/prca.200780090

Author Information

  1. 1

    Discipline of Anatomy and Histology, Bosch Institute, The University of Sydney, Sydney, Australia

  2. 2

    Department of Cardiology, Royal Prince Alfred Hospital, Camperdown, Australia

  3. 3

    Department of Medicine, The University of Sydney, Sydney, Australia

  4. 4

    Department of Clinical Biochemistry, Royal Prince Alfred Hospital, Camperdown, Australia

  5. 5

    Australian Red Cross Blood Service, Sydney, Australia

  6. 6

    Transfusion Medicine and Immunogenetics Research Unit, Central Clinical School, The University of Sydney, Sydney, Australia

  7. 7

    Electron Microscope Unit, Australian Key Centre for Microscopy and Microanalysis, The University of Sydney, Sydney, Australia

*Discipline of Anatomy and Histology, Bosch Institute, The University of Sydney, Sydney 2006, Australia Fax: +61-2-9351-6546

Publication History

  1. Issue published online: 11 JAN 2008
  2. Article first published online: 11 DEC 2007
  3. Manuscript Received: 20 JUN 2007

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Keywords:

  • Antibody microarrays;
  • Atherosclerosis;
  • Cluster of differentiation antigens;
  • Inflammation;
  • Stable and unstable angina

Abstract

There is currently no blood-based test that can rapidly and objectively distinguish between chest pain which is initiated by increased myocardial oxygen demand (stable angina pectoris (SAP)) and chest pain initiated due to decreased coronary blood flow (unstable angina pectoris (UAP)). Since leukocytes play an active role in the progression of coronary artery disease (CAD), we hypothesize these can provide novel markers of SAP and UAP. Here we use a microarray of 82 cluster of differentiation (CD) antibodies (plus controls) to selectively immobilize peripheral blood mononuclear cells. We find that the pattern of leukocyte immobilization from patients with CAD significantly differs from healthy donors. Within the CAD group, 15 SAP patients exhibited significant (p<0.05) changes in 8 of 82 CD antibody spots compared to 19 age-matched healthy blood donors. An additional ten CD antigens differed between healthy donors and patients with UAP (p<0.05). Furthermore, seven CD antibody spots are significantly different between SAP and UAP patients. These preliminary data suggest it is now appropriate to undertake a larger clinical trial to test the hypothesis that these antibody microarrays can monitor the progression from SAP to UAP.

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