Research Article

You have free access to this content

Comprehensive human urine standards for comparability and standardization in clinical proteome analysis

  1. Harald Mischak‡,§,
  2. Walter Kolch†,§,¶,
  3. Michalis Aivaliotis,
  4. David Bouyssié,
  5. Magali Court,
  6. Hassan Dihazi‡,
  7. Gry H. Dihazi,
  8. Julia Franke,
  9. Jérôme Garin‡,
  10. Anne Gonzalez de Peredo,
  11. Alexander Iphöfer,
  12. Lothar Jänsch,
  13. Chrystelle Lacroix,
  14. Manousos Makridakis,
  15. Christophe Masselon‡,
  16. Jochen Metzger,
  17. Bernard Monsarrat‡,
  18. Michal Mrug,
  19. Martin Norling,
  20. Jan Novak,
  21. Andreas Pich,
  22. Andrew Pitt,
  23. Erik Bongcam-Rudloff‡,
  24. Justyna Siwy,
  25. Hitoshi Suzuki,
  26. Visith Thongboonkerd‡,
  27. Li-Shun Wang,
  28. Jérôme Zoidakis,
  29. Petra Zürbig,
  30. Joost P. Schanstra‡,
  31. Antonia Vlahou‡,*

Article first published online: 3 FEB 2010

DOI: 10.1002/prca.200900189

Issue

PROTEOMICS - Clinical Applications

PROTEOMICS - Clinical Applications

Special Issue: Liver Proteomics

Volume 4, Issue 4, pages 464–478, April 2010

Additional Information

How to Cite

Mischak, H., Kolch, W., Aivaliotis, M., Bouyssié, D., Court, M., Dihazi, H., Dihazi, G. H., Franke, J., Garin, J., de Peredo, A. G., Iphöfer, A., Jänsch, L., Lacroix, C., Makridakis, M., Masselon, C., Metzger, J., Monsarrat, B., Mrug, M., Norling, M., Novak, J., Pich, A., Pitt, A., Bongcam-Rudloff, E., Siwy, J., Suzuki, H., Thongboonkerd, V., Wang, L.-S., Zoidakis, J., Zürbig, P., Schanstra, J. P. and Vlahou, A. (2010), Comprehensive human urine standards for comparability and standardization in clinical proteome analysis. Prot. Clin. Appl., 4: 464–478. doi: 10.1002/prca.200900189

Author Information

  1. Mosaiques Diagnostics and Therapeutics AG, Hannover, Germany

  1. †

    Systems Biology Ireland, University College Dublin, Ireland

  1. ‡

    From the European Kidney and Urine Proteomics (EuroKUP) Consortium.

  2. §

    These authors contributed equally to this work.

  3. For other affiliations please see Addendum

*Laboratory of Biotechnology, Biomedical Research Foundation, Academy of Athens, Soranou Efessiou 4, 11527 Athens, Greece Fax: +30-210-65-97-545

Publication History

  1. Issue published online: 26 MAR 2010
  2. Article first published online: 3 FEB 2010
  3. Manuscript Accepted: 26 DEC 2009
  4. Manuscript Revised: 20 NOV 2009
  5. Manuscript Received: 23 SEP 2009

Funded by

  • European Union through InGenious HyperCare. Grant Number: LSHM-C7-2006-037093
  • EuroKUP COST Action. Grant Number: BM0702
  • Agence Nationale pour la Recherche. Grant Number: ANR-07-PHYSIO-004-01
  • NIH grants. Grant Numbers: DK075868, DK078244

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article with Supporting Information (HTML) Get PDF (556K)

Keywords:

  • Clinical proteomics;
  • Proteome;
  • Standard;
  • Urine

Abstract

Purpose: Urine proteomics is emerging as a powerful tool for biomarker discovery. The purpose of this study is the development of a well-characterized “real life” sample that can be used as reference standard in urine clinical proteomics studies.

Experimental design: We report on the generation of male and female urine samples that are extensively characterized by different platforms and methods (CE-MS, LC-MS, LC-MS/MS, 1-D gel analysis in combination with nano-LC MS/MS (using LTQ-FT ultra), and 2-DE-MS) for their proteome and peptidome. In several cases analysis involved a definition of the actual biochemical entities, i.e. proteins/peptides associated with molecular mass and detected PTMs and the relative abundance of these compounds.

Results: The combination of different technologies allowed coverage of a wide mass range revealing the advantages and complementarities of the different technologies. Application of these samples in “inter-laboratory” and “inter-platform” data comparison is also demonstrated.

Conclusions and clinical relevance: These well-characterized urine samples are freely available upon request to enable data comparison especially in the context of biomarker discovery and validation studies. It is also expected that they will provide the basis for the comprehensive characterization of the urinary proteome.

View Full Article with Supporting Information (HTML) Get PDF (556K)