Analysis of serum and plasma identifies differences in molecular coverage, measurement variability, and candidate biomarker selection
Article first published online: 29 JUN 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PROTEOMICS - Clinical Applications
Volume 6, Issue 5-6, pages 297–303, June 2012
How to Cite
Alsaif, M., Guest, P. C., Schwarz, E., Reif, A., Kittel-Schneider, S., Spain, M., Rahmoune, H. and Bahn, S. (2012), Analysis of serum and plasma identifies differences in molecular coverage, measurement variability, and candidate biomarker selection. Prot. Clin. Appl., 6: 297–303. doi: 10.1002/prca.201100061
- Issue published online: 29 JUN 2012
- Article first published online: 29 JUN 2012
- Accepted manuscript online: 29 MAY 2012 02:04AM EST
- Manuscript Accepted: 22 FEB 2012
- Manuscript Revised: 24 JAN 2012
- Manuscript Received: 14 JUL 2011
- Stanley Medical Research Institute and the European Union FP7 SchizDX research programme. Grant Number: 223427
- scholarship from the Ministry of Higher Education
- Multiplex immunoassay;
- Measurement variability;
To compare the use of serum and plasma in multiplex immunoassay analyses of 190 proteins and small molecules, and associated molecular pathways. We also tested whether differences between these biofluids can influence the identification of potential biomarkers in a preliminary study comparing bipolar disorder patients with controls.
Using multiplexed immunoassay analyses, we compared the measurement levels and interindividual variation of 190 proteins and small molecules between serum and plasma collected from 21 healthy individuals. We exemplify how this can impact on the outcome of biomarker discovery studies using a case study of 24 patients with bipolar disorder.
Detection of analytes was similar for serum and plasma, although there were marked differences in measurement variability for 29 proteins and cortisol. When considering the disease cohort we identified six proteins that changed significantly in serum and ten in plasma with an overlap of two proteins.
Conclusions and clinical relevance
In spite of the similarities of coverage on a multiplexed platform for serum and plasma, there were important differences in interindividual variability, which can have significant impact on identifications made in biomarker studies.