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New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells
Article first published online: 29 OCT 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PROTEOMICS - Clinical Applications
Volume 6, Issue 9-10, pages 467–475, October 2012
How to Cite
Huang, R., Yu, M., Li, C.-Y., Zhan, Y.-Q., Xu, W.-X., Xu, F., Ge, C.-h., Li, W. and Yang, X.-M. (2012), New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells. Prot. Clin. Appl., 6: 467–475. doi: 10.1002/prca.201200009
- Issue published online: 29 OCT 2012
- Article first published online: 29 OCT 2012
- Accepted manuscript online: 23 JUL 2012 12:58AM EST
- Manuscript Accepted: 5 JUN 2012
- Manuscript Revised: 20 MAY 2012
- Manuscript Received: 21 FEB 2012
- Major State Basic Research of China. Grant Number: 2010CB911904
- Chinese National Natural Science Fund. Grant Number: 81070392
- Protein complex;
- RNA processing
The eukaryotic cytosolic chaperonin containing TCP-1 (CCT) plays an important role in maintaining cellular homeostasis by assisting the folding of many proteins and is also well known for the critical roles in disease. However, the functions of CCT complex have not been established globally, especially when translocating into nuclear. The purpose of this study is to explore the function of CCT in nuclear and present a strategy in clinical proteomics studies.
Blue native polyacrylamide gel electrophoresis (BN-PAGE) combined with mass spectrometry was applied to separate and identify CCT protein complexes.
We isolated the CCT complex in K562 nucleus and identified a novel CCT complex containing 40 protein components involved in protein folding, RNA processing, apoptosis, and cell metabolism. The interactions between four candidate proteins and CCT were confirmed by immunoblotting. Computational biological analyses and independent biochemical assays validated the overall quality of interactions.
Conclusions and clinical relevance
Our results support clues that CCT might play an unexpected role in various biological processes including RNA processing. And we envision future applications for this system searching for new clues of CCT in disease and readily be applied to the clinic.