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New insights into the functions and localization of nuclear CCT protein complex in K562 leukemia cells

Authors

  • Rui Huang,

    1. Beijing Institute of Radiation Medicine, Beijing, China
    2. Beijing Proteomics Research Center, Beijing, China
    3. State Key Laboratory of Proteomics, Beijing, China
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  • Miao Yu,

    1. Beijing Institute of Radiation Medicine, Beijing, China
    2. Beijing Proteomics Research Center, Beijing, China
    3. State Key Laboratory of Proteomics, Beijing, China
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  • Chang-Yan Li,

    1. Beijing Institute of Radiation Medicine, Beijing, China
    2. Beijing Proteomics Research Center, Beijing, China
    3. State Key Laboratory of Proteomics, Beijing, China
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  • Yi-Qun Zhan,

    1. Beijing Institute of Radiation Medicine, Beijing, China
    2. Beijing Proteomics Research Center, Beijing, China
    3. State Key Laboratory of Proteomics, Beijing, China
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  • Wang-Xiang Xu,

    1. Beijing Institute of Radiation Medicine, Beijing, China
    2. Beijing Proteomics Research Center, Beijing, China
    3. State Key Laboratory of Proteomics, Beijing, China
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  • Feifei Xu,

    1. Beijing Institute of Radiation Medicine, Beijing, China
    2. Beijing Proteomics Research Center, Beijing, China
    3. State Key Laboratory of Proteomics, Beijing, China
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  • Chang-hui Ge,

    1. Beijing Institute of Radiation Medicine, Beijing, China
    2. Beijing Proteomics Research Center, Beijing, China
    3. State Key Laboratory of Proteomics, Beijing, China
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  • Wei Li,

    Corresponding author
    1. Beijing Institute of Radiation Medicine, Beijing, China
    2. State Key Laboratory of Proteomics, Beijing, China
    • Beijing Proteomics Research Center, Beijing, China
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  • Xiao-Ming Yang

    Corresponding author
    1. Beijing Proteomics Research Center, Beijing, China
    2. State Key Laboratory of Proteomics, Beijing, China
    • Beijing Institute of Radiation Medicine, Beijing, China
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  • Colour Online: See the article online to view Figs. 1–4 in colour.

Correspondence: Dr. Xiao Ming Yang, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, P. R. China

E-mail: xmyang2@nic.bmi.ac.cn

Fax: +86-10-68212874

Additional corresponding author: Dr. Wei Li, Email: founder.wei@gmail.com

Abstract

Purpose

The eukaryotic cytosolic chaperonin containing TCP-1 (CCT) plays an important role in maintaining cellular homeostasis by assisting the folding of many proteins and is also well known for the critical roles in disease. However, the functions of CCT complex have not been established globally, especially when translocating into nuclear. The purpose of this study is to explore the function of CCT in nuclear and present a strategy in clinical proteomics studies.

Experimental design

Blue native polyacrylamide gel electrophoresis (BN-PAGE) combined with mass spectrometry was applied to separate and identify CCT protein complexes.

Results

We isolated the CCT complex in K562 nucleus and identified a novel CCT complex containing 40 protein components involved in protein folding, RNA processing, apoptosis, and cell metabolism. The interactions between four candidate proteins and CCT were confirmed by immunoblotting. Computational biological analyses and independent biochemical assays validated the overall quality of interactions.

Conclusions and clinical relevance

Our results support clues that CCT might play an unexpected role in various biological processes including RNA processing. And we envision future applications for this system searching for new clues of CCT in disease and readily be applied to the clinic.

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