These two authors contributed equally to this work.
Proteomic analysis of the aqueous humor in patients with wet age-related macular degeneration
Article first published online: 7 MAY 2013
© 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PROTEOMICS - Clinical Applications
Special Issue: Focus on Vascular Proteomics
Volume 7, Issue 7-8, pages 550–560, August 2013
How to Cite
Yao, J., Liu, X., Yang, Q., Zhuang, M., Wang, F., Chen, X., Hang, H., Zhang, W. and Liu, Q. (2013), Proteomic analysis of the aqueous humor in patients with wet age-related macular degeneration. Prot. Clin. Appl., 7: 550–560. doi: 10.1002/prca.201200012
- Issue published online: 7 AUG 2013
- Article first published online: 7 MAY 2013
- Accepted manuscript online: 18 FEB 2013 04:30AM EST
- Manuscript Accepted: 7 JAN 2013
- Manuscript Revised: 20 DEC 2012
- Manuscript Received: 22 FEB 2012
- National Basic Research Program of China. Grant Number: 2011CB510200
- National Natural Science Foundation of China. Grant Numbers: 30973257, 81170855
- Aqueous humor;
- MALDI-TOF MS/MS;
- Wet age-related macular degeneration
A number of studies have shown that the levels of some proteins in the aqueous humor (AH) are altered and correlate with the mechanisms or prognosis of many eye diseases. To identify the possible mechanisms that lead to the development of wet age-related macular degeneration (AMD), a proteomic analysis of the AH composition from wet AMD patients was performed and compared with that from non-AMD cataract patients.
Six wet AMD and six non-AMD cataract patients were enrolled. A proteomic approach which included two-dimensional electrophoresis coupled with MS and bioinformatics methods were used to identify AH proteins with altered expression in wet AMD compared with non-AMD patients. An ELISA was used to validate the proteomic results.
We separated 78 protein spots and identified 68 that were differently expressed in the wet AMD group and controls. Numerous proteins identified in this study are implicated in inflammation, apoptosis, angiogenesis, and oxidative stress.
Conclusions and clinical relevance
The AH protein composition was significantly different between wet AMD and non-AMD patients. The proteins identified in this study may be potential biomarkers of wet AMD development and might play a role in the mechanisms of wet AMD.