These authors have contributed equally to this work.
Stepwise isolation of human peripheral erythrocytes, T lymphocytes, and monocytes for blood cell proteomics
Article first published online: 24 SEP 2012
© 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PROTEOMICS - Clinical Applications
Volume 6, Issue 9-10, pages 497–501, October 2012
How to Cite
Brosseron, F., May, C., Schoenebeck, B., Tippler, B., Woitalla, D., Kauth, M., Brockmann, K., Meyer, H. E., Berg, D., Bufe, A. and Marcus, K. (2012), Stepwise isolation of human peripheral erythrocytes, T lymphocytes, and monocytes for blood cell proteomics. Prot. Clin. Appl., 6: 497–501. doi: 10.1002/prca.201200032
- Issue published online: 29 OCT 2012
- Article first published online: 24 SEP 2012
- Accepted manuscript online: 28 AUG 2012 11:45AM EST
- Manuscript Accepted: 5 JUL 2012
- Manuscript Revised: 14 JUN 2012
- Manuscript Received: 4 MAY 2012
- Bundesministerium für Bildung und Forschung. Grant Numbers: NGFNplus, FZ 01GS08143
Disclaimer: Supplementary materials have been peer-reviewed but not copyedited.
|prca1418-sup-0001-FigureS1.ppt||205K||Supplementary: Example of purity control by FACS A) FACS analysis of the PBMC fraction. CD3 and CD14 positive cells were separated from unlabeled cells. In this sample, CD3 positive cells made up 46% of the PBMC fraction, while CD14 positive cells accounted only for 3%. B) and C) After subsequent MACS sorting using CD14 and CD3 MACS beads, FACS analysis was repeated for the respective fractions. CD14 positive cells were enriched to 88%, and CD3 positive cells to 95%.|
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